提高对非自体感染的怀疑:鉴定哥斯达黎加的圭亚那利什曼病原虫携带一种与巴西东北部和法属圭亚那病毒基因型相关的利什曼病病毒。

IF 2.5 4区 医学 Q2 PARASITOLOGY
Memorias do Instituto Oswaldo Cruz Pub Date : 2023-01-16 eCollection Date: 2023-01-01 DOI:10.1590/0074-02760220162
Carlos Mata-Somarribas, José Quesada-López, María F Matamoros, César Cervantes-Gómez, Annia Mejía, Karen Chacón, Ivannia Bendig, Roger Campos, Raphael Quesada-Morera, Lilian Motta Cantanhêde, Luiza de Oliveira R Pereira, Elisa Cupolillo
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引用次数: 0

摘要

背景:哥斯达黎加历来忽视利什曼病的预防、控制和研究,包括对全国范围内导致人类疾病的利什曼病种了解有限,对利什曼病RNA病毒完全缺乏了解,而该病毒被认为是导致利什曼病病变恶化和转移的一个因素:本研究的目的是描述哥斯达黎加的一例由吉亚那利什曼病(Viannia)引起的皮肤利什曼病病例,该病例伴有利什曼病病毒 1(LRV1)感染,从而引起人们对该地区输入寄生虫的怀疑:方法:该利什曼病菌株先前在哥斯达黎加通过常规 hsp70 聚合酶链反应-限制性片段长度多态性(PCR-RFLP)进行了鉴定,随后在巴西通过同工酶电泳和桑格测序确定了其特征。采用双重 RT-PCR 方法对 LRV1 进行了筛查,并对获得的片段进行了测序:自 2016 年起,哥斯达黎加开始进行利什曼原虫分离和分型,作为其流行病学监测活动的一部分。截至 2019 年,在已分型的 113 株样本中,只有一株被鉴定为 L. (V.) guyanensis,这也是该国首次确诊该物种。有趣的是,同一菌株的 LRV1 检测结果呈阳性。病毒orf1和2的测序结果显示,该样本与来自巴西东北部和法属圭亚那的其他南美LRV1基因型相似:主要结论:这一发现的独特性使人怀疑它不是自生病毒株。主要结论:这一发现的独特性让人怀疑它不是自生菌株。尽管推测它来自南美,但这一报告表明中美洲利什曼病菌株中也存在上述内生共生体。它在当地扩散的可能性是地区卫生当局在预防和控制利什曼病方面面临的又一个挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Raising the suspicion of a non-autochthonous infection: identification of Leishmania guyanensis from Costa Rica exhibits a Leishmaniavirus related to Brazilian north-east and French Guiana viral genotypes.

Raising the suspicion of a non-autochthonous infection: identification of Leishmania guyanensis from Costa Rica exhibits a Leishmaniavirus related to Brazilian north-east and French Guiana viral genotypes.

Raising the suspicion of a non-autochthonous infection: identification of Leishmania guyanensis from Costa Rica exhibits a Leishmaniavirus related to Brazilian north-east and French Guiana viral genotypes.

Background: Costa Rica has a history of neglecting prevention, control and research of leishmaniasis, including limited understanding on Leishmania species causing human disease across the country and a complete lack of knowledge on the Leishmania RNA virus, described as a factor linked to the worsening and metastasis of leishmanial lesions.

Objectives: The aim of this work was to describe a case of cutaneous leishmaniasis by Leishmania (Viannia) guyanensis, bearing infection with Leishmaniavirus 1 (LRV1) in Costa Rica, raising the suspicion of imported parasites in the region.

Methods: The Leishmania strain was previously identified by routine hsp70 polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Costa Rica and subsequently characterised by isoenzyme electrophoresis and Sanger sequencing in Brazil. Screening for LRV1 was conducted with a dual RT-PCR approach and sequencing of the fragment obtained.

Findings: Since 2016 Costa Rica performs Leishmania isolation and typing as part of its epidemiological surveillance activities. Amongst 113 strains typed until 2019, only one was characterised as a L. (V.) guyanensis, corresponding to the first confirmed report of this species in the country. Interestingly, the same strain tested positive for LRV1. Sequencing of the viral orf1 and 2, clustered this sample with other LRV1 genotypes of South American origin, from the Northeast of Brazil and French Guiana.

Main conclusion: The unique characteristics of this finding raised the suspicion that it was not an autochthonous strain. Notwithstanding its presumed origin, this report points to the occurrence of said endosymbiont in Central American Leishmania strains. The possibility of its local dispersion represents one more challenge faced by regional health authorities in preventing and controlling leishmaniasis.

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来源期刊
CiteScore
5.00
自引率
3.60%
发文量
91
审稿时长
3-8 weeks
期刊介绍: Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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