鼠李叶对大鼠肝、肾、脑组织的急性和亚急性毒性及生化特征。

IF 3.4 Q2 TOXICOLOGY
Melese Shenkut Abebe
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引用次数: 4

摘要

大鼠李是一种传统的药用植物,用于治疗肺炎、扭伤、淋病、风湿病和癣感染,也用于埃塞俄比亚当地饮料的制备。它具有广泛的抗氧化、抗疟疾、抗菌、伤口愈合和抗炎活性。这些活性是由于生物碱、类固醇、三萜、单宁、黄酮类、黄酮类、酚类和苷类的存在。本研究旨在探讨红叶对大鼠肝、肾、脑组织的急性和亚急性毒性及生化特征。在急性毒性研究中,雌性大鼠以5000 mg/kg体重的剂量给药,随访14 d。亚急性毒性实验采用四组大鼠。前3组分别给予250、500、1000 mg/kg体重的红尾草提取物,第4组为对照组。记录了中毒症状、食物摄入和体重。尸检时,进行了器官重量测量以及肝、肾和脑的宏观和微观评估。测定了大鼠不同的临床化学特征。单次口服5000mg /kg大鼠大鼠无死亡,说明LD50大于5000mg /kg体重。给药4周后,对大鼠的食物消耗和体重增加没有不良影响。此外,对肝脏、肾脏和大脑进行的大体检查、组织病理学评估和体重测量均未发现治疗相关的变化。生化分析显示治疗组与对照组之间无显著差异。大鼠连续4周食用白藜芦醇叶可能没有毒性作用。但是,应该对长期用药进行进一步的调查,以获得更大的安全范围。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Acute and Subacute Toxicity of <i>Rhamnus prinoides</i> Leaves on Histopathology of Liver, Kidney, and Brain Tissues, and Biochemical Profile of Rats.

Acute and Subacute Toxicity of <i>Rhamnus prinoides</i> Leaves on Histopathology of Liver, Kidney, and Brain Tissues, and Biochemical Profile of Rats.

Acute and Subacute Toxicity of <i>Rhamnus prinoides</i> Leaves on Histopathology of Liver, Kidney, and Brain Tissues, and Biochemical Profile of Rats.

Acute and Subacute Toxicity of Rhamnus prinoides Leaves on Histopathology of Liver, Kidney, and Brain Tissues, and Biochemical Profile of Rats.

Rhamnus prinoides is used as a traditional medicinal plant to treat pneumonia, sprain, gonorrhea, rheumatism, and ringworm infections as well as for the preparation of local beverages in Ethiopia. It has a widespread antioxidant, antimalarial, antimicrobial, wound healing, and anti-inflammatory activities. These activities are due to the presence of alkaloids, steroids, triterpenes, tannins, flavonoids, flavones, phenols, and glycosides. This study aimed to investigate acute and subacute toxicity of R. prinoides leaves on histopathology of the liver, kidney, and brain tissues, and biochemical profiles of rats. For the acute toxicity study, female rats were treated with R. prinoides at a dose of 5000 mg/kg body weight and followed-up for 14 days. In the subacute toxicity study, four groups of rats were used. The first three groups, respectively, received 250, 500, and 1000 mg/kg body weight of R. prinoides extract and the fourth group was a control group. Signs of toxicity, food intake, and weight was recorded. At necropsy, organ weight measurement and macroscopic and microscopic evaluations of the liver, kidney, and brain were carried out. Different clinical chemistry profiles of rats were also measured. Single-dose oral administration of R. prinoides extract at 5000 mg/kg produced no mortality indicating the LD50 is greater than 5000 mg/kg body weight. A four week administration of R. prinoides extract did not bring deleterious outcomes on the food consumption and weight gain of rats. Moreover, gross examination, histopathological evaluation, and weight measurement conducted on the liver, kidney, and brain did not reveal treatment related changes. The biochemical analysis showed no significant difference between the treatment and control groups. Consumption of R. prinoides leaf for 4 weeks might not have a toxic effect in rats. However, further investigations upon long-term administration should be conducted to have a wider safety margin.

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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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