少量普通离子表面活性剂与不饱和磷脂脂模型膜相互作用的研究

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Alicja Sęk , Paulina Perczyk , Aleksandra Szcześ , Rainhard Machatschek , Paweł Wydro
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引用次数: 2

摘要

为了提供常见阴离子表面活性剂与碱性不饱和磷脂相互作用的基本信息,研究了三阳离子(十二烷基三甲基溴化铵,DTAB;研究了十四烷基三甲基溴化铵(TTAB)和十六烷基三甲基溴化铵(CTAB)和一种阴离子(十二烷基硫酸钠,SDS)表面活性剂对1-棕榈酰-2-油基-甘油-3-磷胆碱(POPC)和1,2-二油基- n-甘油-3-磷胆碱(DOPC)膜性能的影响。研究证明,在已合成的脂质体悬浮液中加入微量离子表面活性剂足以显著改变POPC和DOPC脂质体的zeta电位。这种影响随着表面活性剂浓度、表面活性剂烷基链长度和脂质饱和度的增加而增加。而且,在相同烷基链长的情况下,阴离子表面活性剂比阳离子表面活性剂的影响更大。观察到的结果在使用相应的Langmuir单层进行的研究过程中得到证实,其中讨论了表面压力-平均面积等温线,压缩模量-表面压力依赖关系,单层渗透测试,表面电位-平均分子面积等温线和布鲁斯特角度显微镜。结果发现,表面活性剂的存在使等温线向更大的分子面积偏移,SDS的偏移程度高于DTAB。这种效应随着亚相中表面活性剂浓度的增加而增强。此外,所研究的表面活性剂即使在高表面压力下也能保持在单层中。然而,从BAM图像中没有检测到对POPC和DOPC单层形貌的影响。基于zeta电位计算的脂质体表面电位和表面电荷反映了表面活性剂与脂质层之间的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Studies on the interactions of tiny amounts of common ionic surfactants with unsaturated phosphocholine lipid model membranes

Studies on the interactions of tiny amounts of common ionic surfactants with unsaturated phosphocholine lipid model membranes

In order to provide the fundamental information about the interactions of common anionic surfactants with the basic unsaturated phospholipids the influence of three cationic (dodecyltrimethylammonium bromide, DTAB; tetradecyltrimethylammonium bromide, TTAB and hexadecyltrimethylamonium bromide, CTAB) and one anionic (sodium dodecylsulfate, SDS) surfactants on the properties of the 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) layers was investigated. The studies proved that a tiny amount of the ionic surfactant added to the already synthesized liposome suspension is sufficient to change the zeta potential of the POPC and DOPC liposomes significantly. This impact increases with the surfactant concentration, the alkyl chain length of the surfactant and the degree of lipid saturation. Moreover, this effect is greater for the anionic surfactant than for the cationic one of the same alkyl chain length. The observed findings were confirmed in the course of the research carried out with the use of the corresponding Langmuir monolayers where the surface pressure – mean area isotherms, the compressibility modulus – surface pressure dependences, the monolayer penetration tests, the surface potential – mean molecular area isotherms and Brewster angle microscopy were discussed. It was found that the presence of the surfactants shifts the isotherms towards larger molecular area, to the higher extent for the SDS than DTAB. This effect increases with the increasing surfactant concentration in the subphase. Moreover, the investigated surfactants remain in the monolayer even at high surface pressure. Nevertheless, no effect on the morphology of the POPC and DOPC monolayers was detected from the BAM images. The surface potential and surface charge of the liposomes calculated on the basis of the zeta potential results reflected the interactions between the surfactant and the lipid layers.

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来源期刊
Chemistry and Physics of Lipids
Chemistry and Physics of Lipids 生物-生化与分子生物学
CiteScore
7.60
自引率
2.90%
发文量
50
审稿时长
40 days
期刊介绍: Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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