基于视运动反应的小鼠视敏度自动测量:SKY视光。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
So Yeon Ahn, Eun Hye Jung, Hyunmin Ahn, Jihei Sara Lee, Jeong Hyeon Bak, Eun-do Kim, Ja-Hyun Song, Hae-Sol Shin, Munkhdelger Jamiyansharav, Kyoung Yul Seo
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引用次数: 0

摘要

在啮齿类动物视觉科学研究领域,已经发展了几种测量视觉功能的评估方法。然而,视网膜电图测试、视觉诱发电位测试和迷宫测试等方法具有局限性,因为它们仅测量特定类型细胞的功能,难以量化或需要足够的训练时间。该方法利用光动反射和光动反应,即眼睛和头部的补偿运动来响应视觉场景的变化,成为使用最广泛的方法。然而,这种方法需要训练有素的实验人员,而且耗时。我们发现,初学者和专家的视觉敏锐度测量值有显著差异。在这里,我们提出了一种自动验光程序,“SKY验光”,它可以自动跟踪啮齿动物的视运动反应,以克服现有方法的主观性和冗长的评分过程。为了评价8-12周龄C57BL/6小鼠的SKY验光效果,我们将SKY验光的二项决策与熟练的专家进行比较,得出SKY验光曲线下面积为0.845。对比最终视力,SKY验光与专家的类内相关系数值为0.860(95%可信区间(CI) 0.709 ~ 0.928),专家与初学者的类内相关系数值为0.642 (95% CI 0.292 ~ 0.811)。根据专家测量的视力,SKY验光显示出良好的表现水平和良好的评分协议。使用我们的应用程序,研究人员将能够更准确地测试实验动物的视力,同时节省专门训练的时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Automatic measurement of mouse visual acuity based on optomotor response: SKY optomotry.

In the field of visual science study using rodents, several assessment methods have been developed for measuring visual function. However, methods such as electroretinograms tests, visual evoked potentials tests and maze tests have limitations in that they measure function of only a specific type of cells, are difficult to quantify or require sufficient training time. The method which uses an optokinetic reflex and optomotor response, a compensatory eye and head movement in response to changes in the visual scene, became the most widely used method. However, this method requires highly trained experimenters and is time consuming. We showed that measured visual acuity values are significantly different between beginner and expert. Here we suggest an automated optometry program, 'SKY optomotry', which automatically tracks rodents' optomotor response to overcome subjectivity and the lengthy scoring procedure of the existing method. To evaluate the performance of SKY optomotry using 8-12-week-old C57BL/6 mice we compared the binomial decision of SKY optomotry with a skilled expert, and the area under the curve of SKY optomotry was 0.845. Comparing the final visual acuity, the intraclass correlation coefficient value between SKY optomotry and an expert was 0.860 (95% confidence interval (CI) 0.709-0.928), whereas that between an expert and a beginner was 0.642 (95% CI 0.292-0.811). SKY optomotry showed an excellent level of performance with good inter-rater agreements based on the visual acuity measured by an expert. With the use of our application, researchers will be able to test an experimental animal's eyesight more accurately while saving time on specialized training.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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