采用开顶光片显微镜的Barrett食管无损三维病理学图像图谱。

IF 3.7 3区 医学 Q2 MEDICAL LABORATORY TECHNOLOGY
Deepti M Reddi, Lindsey A Barner, Wynn Burke, Gan Gao, William M Grady, Jonathan T C Liu
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引用次数: 2

摘要

上下文。--:解剖病理学家对切片到载玻片上并在亮视野显微镜下观察的组织样本进行诊断。这种方法对样本具有破坏性,这可能会限制其用于辅助分析,从而为患者管理提供信息。此外,对每个样本相对较少的2D组织切片的主观解释导致病理学家在评估(诊断和分级)各种病变时观察者之间的一致性较低。目标。--:评估厚福尔马林固定的Barrett食管标本的3D病理学数据集,这些标本用开放式光片(OTLS)显微镜无损成像。设计。--:将福尔马林固定、石蜡包埋的Barrett食管样品(N=15)脱蜡,用苏木精-伊红荧光类似物染色,光学清除,并用OTLS显微镜进行无损成像。随后将OTLS显微镜图像与每个样品的存档苏木精-伊红组织学切片进行比较。结果。--:Barrett食管样本,包括小型内窥镜钳活检和内窥镜粘膜切除,在OTLS显微镜和常规光学显微镜之间表现出相似的可分辨结构,物镜高达20倍(×200总放大倍数)。OTLS显微镜生成的3D组织学图像可以提高对筛状和良好形成的腺体形态的辨别能力。此外,OTLS显微镜可以观察到更大量的组织,这使得能够改进对表现出高度空间异质性的临床标本的评估。结论。--:在食道标本中,OTLS显微镜可以生成质量与传统光学显微镜相当的图像,其优点是提供3D信息以增强对腺体形态的评估,并使更多的组织标本能够无损可视化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nondestructive 3D Pathology Image Atlas of Barrett Esophagus With Open-Top Light-Sheet Microscopy.

Context.—: Anatomic pathologists render diagnosis on tissue samples sectioned onto glass slides and viewed under a bright-field microscope. This approach is destructive to the sample, which can limit its use for ancillary assays that can inform patient management. Furthermore, the subjective interpretation of a relatively small number of 2D tissue sections per sample contributes to low interobserver agreement among pathologists for the assessment (diagnosis and grading) of various lesions.

Objective.—: To evaluate 3D pathology data sets of thick formalin-fixed Barrett esophagus specimens imaged nondestructively with open-top light-sheet (OTLS) microscopy.

Design.—: Formalin-fixed, paraffin-embedded Barrett esophagus samples (N = 15) were deparaffinized, stained with a fluorescent analog of hematoxylin-eosin, optically cleared, and imaged nondestructively with OTLS microscopy. The OTLS microscopy images were subsequently compared with archived hematoxylin-eosin histology sections from each sample.

Results.—: Barrett esophagus samples, both small endoscopic forceps biopsies and endoscopic mucosal resections, exhibited similar resolvable structures between OTLS microscopy and conventional light microscopy with up to a ×20 objective (×200 overall magnification). The 3D histologic images generated by OTLS microscopy can enable improved discrimination of cribriform and well-formed gland morphologies. In addition, a much larger amount of tissue is visualized with OTLS microscopy, which enables improved assessment of clinical specimens exhibiting high spatial heterogeneity.

Conclusions.—: In esophageal specimens, OTLS microscopy can generate images comparable in quality to conventional light microscopy, with the advantages of providing 3D information for enhanced evaluation of glandular morphologies and enabling much more of the tissue specimen to be visualized nondestructively.

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来源期刊
CiteScore
9.20
自引率
2.20%
发文量
369
审稿时长
3-8 weeks
期刊介绍: Welcome to the website of the Archives of Pathology & Laboratory Medicine (APLM). This monthly, peer-reviewed journal of the College of American Pathologists offers global reach and highest measured readership among pathology journals. Published since 1926, ARCHIVES was voted in 2009 the only pathology journal among the top 100 most influential journals of the past 100 years by the BioMedical and Life Sciences Division of the Special Libraries Association. Online access to the full-text and PDF files of APLM articles is free.
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