Hui Fan , Jiang Li , Weidong Wu , Rui Chen , Min Yang , Yinggang Zhang , Lulu Cong , Lirong Dai , Yu Deng , Lei Cheng , Shichun Ma
{"title":"一种中等耐酸和空气耐受性的厌氧细菌Acidilutibacter cellobiosedens gen. nov.， sp. nov.属于Acidilutibacteraceae家族。2011年11月，提出了孢子囊菌科菌群的建议。11 .和暖皮菌科;11月。","authors":"Hui Fan , Jiang Li , Weidong Wu , Rui Chen , Min Yang , Yinggang Zhang , Lulu Cong , Lirong Dai , Yu Deng , Lei Cheng , Shichun Ma","doi":"10.1016/j.syapm.2022.126376","DOIUrl":null,"url":null,"abstract":"<div><p><span>A Gram-stain positive, moderately thermophilic, acidotolerant and aerotolerant anaerobic bacterium, designated JN-28 </span><sup>T</sup>, was isolated from the pit mud of Chinese strong-flavor liquor. Growth was observed at 25–50 °C and pH 5.5–8.0 in the presence of 0–25 g l<sup>−1</sup> NaCl (optimally at 45 °C, pH 6.0, without NaCl). Strain JN-28 <sup>T</sup> was heterotrophic, requiring yeast extract for growth. The major cellular fatty acids were <em>iso</em>-C<sub>15:0</sub> and C<sub>14:0</sub><span><span>. The DNA G + C content of genomic DNA was 33.54 mol%. The strain was resistant to </span>vancomycin (10 mg l</span><sup>−1</sup><span><span>). Genome analysis revealed the presence of genes involved in the response to mild acid stress and oxidative stress, and resistance to vancomycin. 16S rRNA gene-based </span>phylogenetic analysis showed that strain JN-28 </span><sup>T</sup> shares ≤ 89.3 % sequence similarity with its closest relatives <em>Sporanaerobacter acetigenes</em> DSM 13106 <sup>T</sup> and other members in the order <em>Tissierellales</em>. Based on phenotypic and phylogenetic characteristics, <em>Acidilutibacter cellobiosedens</em><span> gen. nov., sp. nov. is proposed for the new genus and novel species with the type strain JN-28 </span><sup>T</sup> (=CCAM 418 <sup>T</sup> = JCM 39087 <sup>T</sup><span>). Further phylogenetic and phylogenomic analyses suggested strain JN-28 </span><sup>T</sup> represents a novel family within the order <em>Tissierellales</em>, for which <em>Acidilutibacteraceae</em> fam. nov. is proposed. In addition, the family <em>Tissierellaceae</em> was reclassified, <em>Sporanaerobacteraceae</em> fam. nov. and <em>Tepidimicrobiaceae</em> fam. nov. were formally proposed. Emended description of the family <em>Tissierellaceae</em> is also provided.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Description of a moderately acidotolerant and aerotolerant anaerobic bacterium Acidilutibacter cellobiosedens gen. nov., sp. nov. within the family Acidilutibacteraceae fam. nov., and proposal of Sporanaerobacteraceae fam. nov. and Tepidimicrobiaceae fam. nov.\",\"authors\":\"Hui Fan , Jiang Li , Weidong Wu , Rui Chen , Min Yang , Yinggang Zhang , Lulu Cong , Lirong Dai , Yu Deng , Lei Cheng , Shichun Ma\",\"doi\":\"10.1016/j.syapm.2022.126376\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>A Gram-stain positive, moderately thermophilic, acidotolerant and aerotolerant anaerobic bacterium, designated JN-28 </span><sup>T</sup>, was isolated from the pit mud of Chinese strong-flavor liquor. Growth was observed at 25–50 °C and pH 5.5–8.0 in the presence of 0–25 g l<sup>−1</sup> NaCl (optimally at 45 °C, pH 6.0, without NaCl). Strain JN-28 <sup>T</sup> was heterotrophic, requiring yeast extract for growth. The major cellular fatty acids were <em>iso</em>-C<sub>15:0</sub> and C<sub>14:0</sub><span><span>. The DNA G + C content of genomic DNA was 33.54 mol%. The strain was resistant to </span>vancomycin (10 mg l</span><sup>−1</sup><span><span>). Genome analysis revealed the presence of genes involved in the response to mild acid stress and oxidative stress, and resistance to vancomycin. 16S rRNA gene-based </span>phylogenetic analysis showed that strain JN-28 </span><sup>T</sup> shares ≤ 89.3 % sequence similarity with its closest relatives <em>Sporanaerobacter acetigenes</em> DSM 13106 <sup>T</sup> and other members in the order <em>Tissierellales</em>. Based on phenotypic and phylogenetic characteristics, <em>Acidilutibacter cellobiosedens</em><span> gen. nov., sp. nov. is proposed for the new genus and novel species with the type strain JN-28 </span><sup>T</sup> (=CCAM 418 <sup>T</sup> = JCM 39087 <sup>T</sup><span>). Further phylogenetic and phylogenomic analyses suggested strain JN-28 </span><sup>T</sup> represents a novel family within the order <em>Tissierellales</em>, for which <em>Acidilutibacteraceae</em> fam. nov. is proposed. In addition, the family <em>Tissierellaceae</em> was reclassified, <em>Sporanaerobacteraceae</em> fam. nov. and <em>Tepidimicrobiaceae</em> fam. nov. were formally proposed. Emended description of the family <em>Tissierellaceae</em> is also provided.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202022000832\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202022000832","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Description of a moderately acidotolerant and aerotolerant anaerobic bacterium Acidilutibacter cellobiosedens gen. nov., sp. nov. within the family Acidilutibacteraceae fam. nov., and proposal of Sporanaerobacteraceae fam. nov. and Tepidimicrobiaceae fam. nov.
A Gram-stain positive, moderately thermophilic, acidotolerant and aerotolerant anaerobic bacterium, designated JN-28 T, was isolated from the pit mud of Chinese strong-flavor liquor. Growth was observed at 25–50 °C and pH 5.5–8.0 in the presence of 0–25 g l−1 NaCl (optimally at 45 °C, pH 6.0, without NaCl). Strain JN-28 T was heterotrophic, requiring yeast extract for growth. The major cellular fatty acids were iso-C15:0 and C14:0. The DNA G + C content of genomic DNA was 33.54 mol%. The strain was resistant to vancomycin (10 mg l−1). Genome analysis revealed the presence of genes involved in the response to mild acid stress and oxidative stress, and resistance to vancomycin. 16S rRNA gene-based phylogenetic analysis showed that strain JN-28 T shares ≤ 89.3 % sequence similarity with its closest relatives Sporanaerobacter acetigenes DSM 13106 T and other members in the order Tissierellales. Based on phenotypic and phylogenetic characteristics, Acidilutibacter cellobiosedens gen. nov., sp. nov. is proposed for the new genus and novel species with the type strain JN-28 T (=CCAM 418 T = JCM 39087 T). Further phylogenetic and phylogenomic analyses suggested strain JN-28 T represents a novel family within the order Tissierellales, for which Acidilutibacteraceae fam. nov. is proposed. In addition, the family Tissierellaceae was reclassified, Sporanaerobacteraceae fam. nov. and Tepidimicrobiaceae fam. nov. were formally proposed. Emended description of the family Tissierellaceae is also provided.
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Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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