Ca2+感应受体-瞬时受体电位介导的Ca2+内流对弯曲细胞外酸度的敏感性。3个内皮细胞。

IF 1.4 4区 医学 Q4 PHYSIOLOGY
Iat-Lon Leong, Chung-Ming Yu, Lian-Ru Shiao, Paul Chan, King-Chuen Wu, Yuk-Man Leung
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引用次数: 0

摘要

Ca2+感应受体(CaSRs)是由细胞外Ca2+浓度升高激活的G蛋白偶联受体。在我们之前的工作中,我们发现CaSR在小鼠脑内皮细胞(EC)中的蛋白表达和功能表达(bEND.3);CaSR反应(高Ca2+引起的细胞质[Ca2+]升高)不受磷脂酶C抑制的影响,但部分涉及通过瞬时受体电位V1 (TRPV1)通道的Ca2+内流。在这项工作中,我们研究了细胞外酸度是否影响高(3mm) Ca2+ (CaSR激动剂)、3mm精胺(CaSR激动剂)和10mm cinacalcet (CaSR的阳性变构调节剂)引发的CaSR介导的Ca2+内流。胞外酸中毒(pH 6.8和pH 6.0)强烈抑制由高Ca2+,精胺和cinacalcet引发的胞浆[Ca2+]升高;酸中毒还能抑制高Ca2+和钙离子刺激的Mn2+内流。然而,嘌呤受体触发的Ca2+反应不受酸中毒的抑制。细胞外酸度也不影响膜电位,表明抑制casr介导的Ca2+内流的酸度不是由于Ca2+的电驱动力降低。我们的研究结果表明,Ca2+通过弯曲的假定的CaSR-TRP复合物流入EC对胞外pH值敏感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sensitivity of Ca2+-sensing receptor-transient receptor potential-mediated Ca2+ influx to extracellular acidity in bEND.3 endothelial cells.

Ca2+-sensing receptors (CaSRs) are G protein-coupled receptors activated by elevated concentrations of extracellular Ca2+. In our previous works, we showed protein and functional expression of CaSR in mouse cerebral endothelial cell (EC) (bEND.3); the CaSR response (high Ca2+-elicited cytosolic [Ca2+] elevation) was unaffected by suppression of phospholipase C but in part involved Ca2+ influx through transient receptor potential V1 (TRPV1) channels. In this work, we investigated if extracellular acidity affected CaSR-mediated Ca2+ influx triggered by high (3 mM) Ca2+ (CaSR agonist), 3 mM spermine (CaSR agonist), and 10 mM cinacalcet (positive allosteric modulator of CaSR). Extracellular acidosis (pH 6.8 and pH 6.0) strongly suppressed cytosolic [Ca2+] elevation triggered by high Ca2+, spermine, and cinacalcet; acidosis also inhibited Mn2+ influx stimulated by high Ca2+ and cinacalcet. Purinoceptor-triggered Ca2+ response, however, was not suppressed by acidosis. Extracellular acidity also did not affect membrane potential, suggesting suppressed CaSR-mediated Ca2+ influx in acidity did not result from the reduced electrical driving force for Ca2+. Our results suggest Ca2+ influx through a putative CaSR-TRP complex in bEND.3 EC was sensitive to extracellular pH.

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来源期刊
CiteScore
2.30
自引率
5.60%
发文量
36
审稿时长
6-12 weeks
期刊介绍: Chinese Journal of Physiology is a multidisciplinary open access journal. Chinese Journal of Physiology (CJP) publishes high quality original research papers in physiology and pathophysiology by authors all over the world. CJP welcomes submitted research papers in all aspects of physiology science in the molecular, cellular, tissue and systemic levels. Multidisciplinary sciences with a focus to understand the role of physiology in health and disease are also encouraged. Chinese Journal of Physiology accepts fourfold article types: Original Article, Review Article (Mini-Review included), Short Communication, and Editorial. There is no cost for readers to access the full-text contents of publications.
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