{"title":"以 Tetramorium tsushimae(膜翅目:蚁科)为蚁群水平口服毒性试验中的蚂蚁控制剂对进口红火蚁 Solenopsis invicta(膜翅目:蚁科)建立后的控制效率","authors":"Hironori Sakamoto, Koichi Goka","doi":"10.1007/s13355-022-00800-x","DOIUrl":null,"url":null,"abstract":"<div><p>The red imported fire ant <i>Solenopsis invicta</i> Buren (Hymenoptera: Formicidae) causes serious damage worldwide as an invasive alien species. The species has expanded its range to the Pacific Rim since 2000s and Japan has faced its multiple introductions since 2017. While colony-level control methods are urgently needed, testing living colonies of the unestablished species is challenging especially due to various restrictions under the COVID-19 pandemic. Here, we proposed alternative long-term toxicity assays using artificial colonies of <i>Tetramorium tsushimae</i> Emery (Hymenoptera: Formicidae), a Japanese native species belonging to the same subfamily (Myrmicinae) as <i>S. invicta</i>. We conducted an acute toxicity test to determine if <i>T. tsushimae</i> is a suitable substitute for <i>S. invicta</i> using fipronil and found the LD<sub>50</sub> value in <i>T. tsushimae</i> was close to that in <i>S. invicta</i>. Then, we conducted the long-term toxicity test with fipronil and two insect growth regulators (pyriproxyfen and etoxazole) using artificial colonies of <i>T. tsushimae</i>. All workers and larvae in the fipronil-treated colonies died within 3 days of treatment initiation. Emergence of new workers was observed after 18 days in the etoxazole-treated and control colonies, but not in the pyriproxyfen-treated colonies. We concluded that fipronil was the most promising insecticide for post-establishment control, and pyriproxyfen was effective as a toxic-bait agent for colony-level control.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-022-00800-x.pdf","citationCount":"0","resultStr":"{\"title\":\"Efficiency of ant-control agents in colony-level oral toxicity tests using Tetramorium tsushimae (Hymenoptera: Formicidae) for post-establishment control of the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae)\",\"authors\":\"Hironori Sakamoto, Koichi Goka\",\"doi\":\"10.1007/s13355-022-00800-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The red imported fire ant <i>Solenopsis invicta</i> Buren (Hymenoptera: Formicidae) causes serious damage worldwide as an invasive alien species. The species has expanded its range to the Pacific Rim since 2000s and Japan has faced its multiple introductions since 2017. While colony-level control methods are urgently needed, testing living colonies of the unestablished species is challenging especially due to various restrictions under the COVID-19 pandemic. Here, we proposed alternative long-term toxicity assays using artificial colonies of <i>Tetramorium tsushimae</i> Emery (Hymenoptera: Formicidae), a Japanese native species belonging to the same subfamily (Myrmicinae) as <i>S. invicta</i>. We conducted an acute toxicity test to determine if <i>T. tsushimae</i> is a suitable substitute for <i>S. invicta</i> using fipronil and found the LD<sub>50</sub> value in <i>T. tsushimae</i> was close to that in <i>S. invicta</i>. Then, we conducted the long-term toxicity test with fipronil and two insect growth regulators (pyriproxyfen and etoxazole) using artificial colonies of <i>T. tsushimae</i>. All workers and larvae in the fipronil-treated colonies died within 3 days of treatment initiation. Emergence of new workers was observed after 18 days in the etoxazole-treated and control colonies, but not in the pyriproxyfen-treated colonies. We concluded that fipronil was the most promising insecticide for post-establishment control, and pyriproxyfen was effective as a toxic-bait agent for colony-level control.</p></div>\",\"PeriodicalId\":8551,\"journal\":{\"name\":\"Applied Entomology and Zoology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s13355-022-00800-x.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Entomology and Zoology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13355-022-00800-x\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Entomology and Zoology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13355-022-00800-x","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Efficiency of ant-control agents in colony-level oral toxicity tests using Tetramorium tsushimae (Hymenoptera: Formicidae) for post-establishment control of the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae)
The red imported fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae) causes serious damage worldwide as an invasive alien species. The species has expanded its range to the Pacific Rim since 2000s and Japan has faced its multiple introductions since 2017. While colony-level control methods are urgently needed, testing living colonies of the unestablished species is challenging especially due to various restrictions under the COVID-19 pandemic. Here, we proposed alternative long-term toxicity assays using artificial colonies of Tetramorium tsushimae Emery (Hymenoptera: Formicidae), a Japanese native species belonging to the same subfamily (Myrmicinae) as S. invicta. We conducted an acute toxicity test to determine if T. tsushimae is a suitable substitute for S. invicta using fipronil and found the LD50 value in T. tsushimae was close to that in S. invicta. Then, we conducted the long-term toxicity test with fipronil and two insect growth regulators (pyriproxyfen and etoxazole) using artificial colonies of T. tsushimae. All workers and larvae in the fipronil-treated colonies died within 3 days of treatment initiation. Emergence of new workers was observed after 18 days in the etoxazole-treated and control colonies, but not in the pyriproxyfen-treated colonies. We concluded that fipronil was the most promising insecticide for post-establishment control, and pyriproxyfen was effective as a toxic-bait agent for colony-level control.
期刊介绍:
Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.