维生素D对脂多糖所致大鼠心血管损伤的影响。

IF 1.2 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS
Narges Marefati, Farimah Beheshti, Akbar Anaeigoudari, Fatemeh Alipour, Reyhaneh Shafieian, Fatemeh Akbari, Maryam Pirasteh, Maryam Mahmoudabady, Hossein Salmani, Sara Mawdodi, Mahmoud Hosseini
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引用次数: 0

摘要

炎症和氧化应激与心血管疾病有关。维生素D (Vit D)具有抗氧化和抗炎特性。本研究以脂多糖(LPS)注射大鼠为实验对象,研究Vit D对心肌纤维化、炎症及心血管组织氧化应激指标的影响。方法:将大鼠分为5组,治疗2周。对照组:用培养液(生理盐水中添加吐温-80)代替Vit D,用生理盐水代替LPS, LPS:用1 mg/kg LPS处理,用培养液代替Vit D, LPS-Vit D组:在LPS的基础上分别给予3个剂量的Vit D(100、1000、10000 IU/kg)。Vit D溶解于添加吐温-80(终浓度0.1%)的生理盐水中,LPS溶解于生理盐水中。计数白细胞计数。测定血清、主动脉和心脏的氧化应激标志物。心肌组织纤维化也用马松三色染色法估计。结果:脂多糖组WBC、丙二醛(MDA)含量高于对照组,硫醇含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)含量低于对照组(ppppppppppp)。结论:维生素D对心血管有保护作用。这种心血管保护可归因于维生素D的抗氧化和抗炎特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats.

The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats.

The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats.

The effects of vitamin D on cardiovascular damage induced by lipopolysaccharides in rats.

Introduction: Inflammation and oxidative stress are contributed to cardiovascular diseases. Vitamin D (Vit D) has antioxidant and anti-inflammatory properties. In the current research, the effect of Vit D on cardiac fibrosis and inflammation, and oxidative stress indicators in cardiovascular tissues was studied in lipopolysaccharides(LPS) injected rats.

Methods: Rats were distributed into 5 groups and were treated for 2 weeks. Control: received vehicle(saline supplemented with tween-80) instead of Vit D and saline instead of LPS, LPS: treated by 1 mg/kg of LPS and was given vehicle instead of Vit D, LPS-Vit D groups: received 3 doses of Vit D (100, 1000, and 10000 IU/kg) of Vit D in addition to LPS. Vit D was dissolved in saline supplemented with tween-80 (final concentration 0.1%) and LPS was dissolved in saline. The white blood cell (WBC) was counted. Oxidative stress markers were determined in serum, aorta, and heart. Cardiac tissue fibrosis was also estimated using Masson's trichrome staining method.

Results: WBC and malondialdehyde (MDA) were higher in the LPS group than the control group, whereas the thiol content, superoxide dismutase (SOD), and catalase (CAT) were lower in the LPS group than the control group (P<0.01 and P<0.001). Administration of Vit D decreased WBC (P<0.001) and MDA (P<0.05 and P<0.001) while enhanced thiol (dose 10000 IU/Kg) (P<0.001), SOD (dose 10000 IU/kg) (P<0.001), and CAT (P<0.05 and P<0.001) compared to the LPS group. All doses of Vit D also decreased cardiac fibrosis compared to the LPS group (P<0.001).

Conclusion: Vit D protected the cardiovascular against the detrimental effect of LPS. This cardiovascular protection can be attributed to the antioxidant and anti-inflammatory properties of Vit D.

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来源期刊
Journal of Cardiovascular and Thoracic Research
Journal of Cardiovascular and Thoracic Research CARDIAC & CARDIOVASCULAR SYSTEMS-
CiteScore
2.00
自引率
0.00%
发文量
22
审稿时长
7 weeks
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