Yong Liu , Jing Xin , Shengnan Zhang , Qingmei Li , Wenying Wang , Ji Chen , Xin Ming , Xiaoqing Wu , Xinyan Cao , Wei Cui , Hongcheng Wang , Wenyong Li
{"title":"BCL2L10在小鼠着床前发育中的表达模式及生物学功能","authors":"Yong Liu , Jing Xin , Shengnan Zhang , Qingmei Li , Wenying Wang , Ji Chen , Xin Ming , Xiaoqing Wu , Xinyan Cao , Wei Cui , Hongcheng Wang , Wenyong Li","doi":"10.1016/j.gep.2022.119285","DOIUrl":null,"url":null,"abstract":"<div><p><span>BCL2-like 10 (BCL2L10) is abundantly expressed in mammalian oocytes and plays a crucial role in the completion of oocyte meiosis. However, the expression patterns of BCL2L10 and its biological functions during preimplantation development have not been well characterized. Here, we investigated the spatiotemporal expressions of </span><em>Bcl2l10</em><span> during mouse preimplantation development using RT-qPCR and immunofluorescence and its biological function using siRNA<span> and morpholino injection into pronuclear embryos. Results from RT-qPCR showed that </span></span><em>Bcl2l10</em><span> was highly expressed in the metaphase Ⅱ-stage oocytes and pronuclear-stage embryos, but expression markedly decreased from the two-cell stage onwards and was no longer detected at the four-cell stage and beyond. Immunofluorescence staining showed that BCL2L10 was detectable throughout preimplantation development and localized in the cytoplasm and nuclei. Knocking down </span><em>Bcl2l10</em><span> resulted in a reduced blastocyst formation rate (</span><em>P</em> < 0.01) and decreased expression of OCT4, NANOG, and SOX17 (<em>P</em> < 0.05). We concluded that the role of BCL2L10 is strongly associated with developmental competence of preimplantation mouse embryos.</p></div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"46 ","pages":"Article 119285"},"PeriodicalIF":1.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Expression patterns and biological function of BCL2L10 during mouse preimplantation development\",\"authors\":\"Yong Liu , Jing Xin , Shengnan Zhang , Qingmei Li , Wenying Wang , Ji Chen , Xin Ming , Xiaoqing Wu , Xinyan Cao , Wei Cui , Hongcheng Wang , Wenyong Li\",\"doi\":\"10.1016/j.gep.2022.119285\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>BCL2-like 10 (BCL2L10) is abundantly expressed in mammalian oocytes and plays a crucial role in the completion of oocyte meiosis. However, the expression patterns of BCL2L10 and its biological functions during preimplantation development have not been well characterized. Here, we investigated the spatiotemporal expressions of </span><em>Bcl2l10</em><span> during mouse preimplantation development using RT-qPCR and immunofluorescence and its biological function using siRNA<span> and morpholino injection into pronuclear embryos. Results from RT-qPCR showed that </span></span><em>Bcl2l10</em><span> was highly expressed in the metaphase Ⅱ-stage oocytes and pronuclear-stage embryos, but expression markedly decreased from the two-cell stage onwards and was no longer detected at the four-cell stage and beyond. Immunofluorescence staining showed that BCL2L10 was detectable throughout preimplantation development and localized in the cytoplasm and nuclei. Knocking down </span><em>Bcl2l10</em><span> resulted in a reduced blastocyst formation rate (</span><em>P</em> < 0.01) and decreased expression of OCT4, NANOG, and SOX17 (<em>P</em> < 0.05). We concluded that the role of BCL2L10 is strongly associated with developmental competence of preimplantation mouse embryos.</p></div>\",\"PeriodicalId\":55598,\"journal\":{\"name\":\"Gene Expression Patterns\",\"volume\":\"46 \",\"pages\":\"Article 119285\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene Expression Patterns\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567133X22000552\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Expression Patterns","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567133X22000552","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Expression patterns and biological function of BCL2L10 during mouse preimplantation development
BCL2-like 10 (BCL2L10) is abundantly expressed in mammalian oocytes and plays a crucial role in the completion of oocyte meiosis. However, the expression patterns of BCL2L10 and its biological functions during preimplantation development have not been well characterized. Here, we investigated the spatiotemporal expressions of Bcl2l10 during mouse preimplantation development using RT-qPCR and immunofluorescence and its biological function using siRNA and morpholino injection into pronuclear embryos. Results from RT-qPCR showed that Bcl2l10 was highly expressed in the metaphase Ⅱ-stage oocytes and pronuclear-stage embryos, but expression markedly decreased from the two-cell stage onwards and was no longer detected at the four-cell stage and beyond. Immunofluorescence staining showed that BCL2L10 was detectable throughout preimplantation development and localized in the cytoplasm and nuclei. Knocking down Bcl2l10 resulted in a reduced blastocyst formation rate (P < 0.01) and decreased expression of OCT4, NANOG, and SOX17 (P < 0.05). We concluded that the role of BCL2L10 is strongly associated with developmental competence of preimplantation mouse embryos.
期刊介绍：
Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include:
-In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression
-Temporal studies of large gene sets during development
-Transgenic studies to study cell lineage in tissue formation
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