综合多组学揭示了绿原酸在改善免疫应激肉鸡生长性能和免疫功能方面的有益作用

IF 6.3
Huawei Liu , Xuemin Li , Kai Zhang, Xiaoguo Lv, Quanwei Zhang, Peng Chen, Yang Wang, Jinshan Zhao
{"title":"综合多组学揭示了绿原酸在改善免疫应激肉鸡生长性能和免疫功能方面的有益作用","authors":"Huawei Liu ,&nbsp;Xuemin Li ,&nbsp;Kai Zhang,&nbsp;Xiaoguo Lv,&nbsp;Quanwei Zhang,&nbsp;Peng Chen,&nbsp;Yang Wang,&nbsp;Jinshan Zhao","doi":"10.1016/j.aninu.2023.05.009","DOIUrl":null,"url":null,"abstract":"<div><p>Intensive production can cause immunological stress in commercial broilers. Chlorogenic acid (CGA) regulates the intestinal microbiota, barrier function, and immune function in chickens. As complex interrelations regulate the dynamic interplay between gut microbiota, the host, and diverse health outcomes, the aim of this study was to elucidate the immunoregulatory mechanisms of CGA using multi-omics approaches. A total of 240 one-day-old male broilers were assigned to a 2 × 2 factorial design with 2 CGA levels (0 or 500 mg/kg) either with or without dexamethasone (DEX) injection for a 21-day experimental period. Therefore, there were 4 dietary treatments: control, DEX, CGA, and DEX + CGA, with 6 replicates per treatment. CGA supplementation improved (<em>P</em> &lt; 0.05) growth performance, jejunal morphology, jejunal barrier function, and immune function in DEX-treated broilers. Moreover, in DEX + CGA-treated broilers, the increase in gut microbiome diversity (<em>P</em> &lt; 0.05) was consistent with a change in taxonomic composition, especially in the Clostridiales vadin BB60_group. Additionally, the levels of short-chain fatty acids increased remarkably (<em>P</em> &lt; 0.01) after CGA supplementation. This was consistent with the Kyoto Encyclopedia of Genes and Genomes analysis results that the “pyruvate fermentation to butanoate” pathway was more enriched (<em>P</em> &lt; 0.01) in the DEX + CGA group than in the DEX group. Proteomics revealed that CGA treatment increased the expression of several health-promoting proteins, thymosin beta (TMSB4X) and legumain (LGMN), which were verified by multiple reaction monitoring. Metabolomics revealed that CGA treatment increased the expression of health-promoting metabolites (2,4-dihydroxy benzoic acid and homogentisic acid). Proteomic and metabolic analyses showed that CGA treatment regulated the peroxisome proliferator-activated receptor (PPAR) and mitogen-activated protein kinase (MAPK) pathways. Western blotting results support these findings. Pearson’s correlation analyses showed correlations (<em>P</em> &lt; 0.01) between altered immune function, jejunal barrier function, different microbiota, proteins, and metabolites parameters. Overall, our data indicate that CGA treatment increased growth performance and improved the immunological functions of DEX-treated broilers by regulating gut microbiota and the PPAR and MAPK pathways. The results offer novel insights into a CGA-mediated improvement in immune function and intestinal health.</p></div>","PeriodicalId":62604,"journal":{"name":"Animal Nutrition","volume":null,"pages":null},"PeriodicalIF":6.3000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/66/64/main.PMC10448031.pdf","citationCount":"1","resultStr":"{\"title\":\"Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers\",\"authors\":\"Huawei Liu ,&nbsp;Xuemin Li ,&nbsp;Kai Zhang,&nbsp;Xiaoguo Lv,&nbsp;Quanwei Zhang,&nbsp;Peng Chen,&nbsp;Yang Wang,&nbsp;Jinshan Zhao\",\"doi\":\"10.1016/j.aninu.2023.05.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Intensive production can cause immunological stress in commercial broilers. Chlorogenic acid (CGA) regulates the intestinal microbiota, barrier function, and immune function in chickens. As complex interrelations regulate the dynamic interplay between gut microbiota, the host, and diverse health outcomes, the aim of this study was to elucidate the immunoregulatory mechanisms of CGA using multi-omics approaches. A total of 240 one-day-old male broilers were assigned to a 2 × 2 factorial design with 2 CGA levels (0 or 500 mg/kg) either with or without dexamethasone (DEX) injection for a 21-day experimental period. Therefore, there were 4 dietary treatments: control, DEX, CGA, and DEX + CGA, with 6 replicates per treatment. CGA supplementation improved (<em>P</em> &lt; 0.05) growth performance, jejunal morphology, jejunal barrier function, and immune function in DEX-treated broilers. Moreover, in DEX + CGA-treated broilers, the increase in gut microbiome diversity (<em>P</em> &lt; 0.05) was consistent with a change in taxonomic composition, especially in the Clostridiales vadin BB60_group. Additionally, the levels of short-chain fatty acids increased remarkably (<em>P</em> &lt; 0.01) after CGA supplementation. This was consistent with the Kyoto Encyclopedia of Genes and Genomes analysis results that the “pyruvate fermentation to butanoate” pathway was more enriched (<em>P</em> &lt; 0.01) in the DEX + CGA group than in the DEX group. Proteomics revealed that CGA treatment increased the expression of several health-promoting proteins, thymosin beta (TMSB4X) and legumain (LGMN), which were verified by multiple reaction monitoring. Metabolomics revealed that CGA treatment increased the expression of health-promoting metabolites (2,4-dihydroxy benzoic acid and homogentisic acid). Proteomic and metabolic analyses showed that CGA treatment regulated the peroxisome proliferator-activated receptor (PPAR) and mitogen-activated protein kinase (MAPK) pathways. Western blotting results support these findings. Pearson’s correlation analyses showed correlations (<em>P</em> &lt; 0.01) between altered immune function, jejunal barrier function, different microbiota, proteins, and metabolites parameters. Overall, our data indicate that CGA treatment increased growth performance and improved the immunological functions of DEX-treated broilers by regulating gut microbiota and the PPAR and MAPK pathways. The results offer novel insights into a CGA-mediated improvement in immune function and intestinal health.</p></div>\",\"PeriodicalId\":62604,\"journal\":{\"name\":\"Animal Nutrition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/66/64/main.PMC10448031.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Nutrition\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405654523000677\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Nutrition","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405654523000677","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

集约生产会导致商品肉鸡的免疫应激。绿原酸(CGA)调节鸡的肠道微生物群、屏障功能和免疫功能。由于复杂的相互关系调节肠道微生物群、宿主和不同健康结果之间的动态相互作用,本研究的目的是使用多组学方法阐明CGA的免疫调节机制。将240只一日龄雄性肉鸡分为2×2析因设计,2个CGA水平(0或500mg/kg),注射或不注射地塞米松(DEX),为期21天。因此,有4个日粮处理:对照、DEX、CGA和DEX+CGA,每个处理有6个重复。添加CGA改善了(P<;0.05)DEX处理肉鸡的生长性能、空肠形态、空肠屏障功能和免疫功能。此外,在DEX+CGA处理的肉鸡中,肠道微生物组多样性的增加(P<;0.05)与分类学组成的变化一致,尤其是在梭状芽孢杆菌-vadin BB60_组中。此外,补充CGA后,短链脂肪酸的水平显著增加(P<0.01)。这与京都基因和基因组百科全书的分析结果一致,即DEX+CGA组中的“丙酮酸发酵到丁酸”途径比DEX组中更富集(P<;0.01)。蛋白质组学显示,CGA治疗增加了几种促进健康的蛋白质,胸腺肽β(TMSB4X)和legumain(LGMN)的表达,这些蛋白质已通过多种反应监测得到验证。代谢组学显示,CGA处理增加了健康促进代谢产物(2,4-二羟基苯甲酸和匀浆酸)的表达。蛋白质组学和代谢分析表明,CGA处理调节过氧化物酶体增殖物激活受体(PPAR)和丝裂原激活蛋白激酶(MAPK)途径。蛋白质印迹结果支持这些发现。Pearson相关性分析显示,免疫功能改变、空肠屏障功能、不同微生物群、蛋白质和代谢产物参数之间存在相关性(P<;0.01)。总体而言,我们的数据表明,CGA处理通过调节肠道微生物群以及PPAR和MAPK途径,提高了DEX处理肉鸡的生长性能,并改善了其免疫功能。这一结果为CGA介导的免疫功能和肠道健康改善提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers

Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers

Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers

Integrated multi-omics reveals the beneficial role of chlorogenic acid in improving the growth performance and immune function of immunologically stressed broilers

Intensive production can cause immunological stress in commercial broilers. Chlorogenic acid (CGA) regulates the intestinal microbiota, barrier function, and immune function in chickens. As complex interrelations regulate the dynamic interplay between gut microbiota, the host, and diverse health outcomes, the aim of this study was to elucidate the immunoregulatory mechanisms of CGA using multi-omics approaches. A total of 240 one-day-old male broilers were assigned to a 2 × 2 factorial design with 2 CGA levels (0 or 500 mg/kg) either with or without dexamethasone (DEX) injection for a 21-day experimental period. Therefore, there were 4 dietary treatments: control, DEX, CGA, and DEX + CGA, with 6 replicates per treatment. CGA supplementation improved (P < 0.05) growth performance, jejunal morphology, jejunal barrier function, and immune function in DEX-treated broilers. Moreover, in DEX + CGA-treated broilers, the increase in gut microbiome diversity (P < 0.05) was consistent with a change in taxonomic composition, especially in the Clostridiales vadin BB60_group. Additionally, the levels of short-chain fatty acids increased remarkably (P < 0.01) after CGA supplementation. This was consistent with the Kyoto Encyclopedia of Genes and Genomes analysis results that the “pyruvate fermentation to butanoate” pathway was more enriched (P < 0.01) in the DEX + CGA group than in the DEX group. Proteomics revealed that CGA treatment increased the expression of several health-promoting proteins, thymosin beta (TMSB4X) and legumain (LGMN), which were verified by multiple reaction monitoring. Metabolomics revealed that CGA treatment increased the expression of health-promoting metabolites (2,4-dihydroxy benzoic acid and homogentisic acid). Proteomic and metabolic analyses showed that CGA treatment regulated the peroxisome proliferator-activated receptor (PPAR) and mitogen-activated protein kinase (MAPK) pathways. Western blotting results support these findings. Pearson’s correlation analyses showed correlations (P < 0.01) between altered immune function, jejunal barrier function, different microbiota, proteins, and metabolites parameters. Overall, our data indicate that CGA treatment increased growth performance and improved the immunological functions of DEX-treated broilers by regulating gut microbiota and the PPAR and MAPK pathways. The results offer novel insights into a CGA-mediated improvement in immune function and intestinal health.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信