设计与合成4-叠氮-磷脂酰肌醇作为糖基磷脂酰肌醇在细胞上代谢工程的潜在探针

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-01-01 DOI:10.1080/07328303.2022.2077358

Kendall C. Craig , Zhongwu Guo

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引用次数: 2

摘要

以1,2-二硬脂酰-sn-甘油和d -葡萄糖为原料，通过19步有效地合成了一种二酰基磷脂酰肌醇(PI)衍生物，其叠氮化物与肌醇c4位置相连，具有最长的线性序列，总收率约为1%。该化合物被设计为糖基磷脂酰肌醇(GPI)锚定的生物合成前体。它的叠氮化物可以通过生物相容性点击反应进一步修饰引入其他分子标签。因此，它可以作为细胞表面GPI锚定物和GPI锚定蛋白代谢工程的有用探针。下载:下载高清图片(26KB)下载:下载全尺寸图片

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Design and synthesis of 4-azido-phosphatidylinositol as a potential probe for metabolic engineering of glycosylphosphatidylinositol on cells

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Design and synthesis of 4-azido-phosphatidylinositol as a potential probe for metabolic engineering of glycosylphosphatidylinositol on cells

A diacyl phosphatidylinositol (PI) derivative with an azide linked to its inositol C4-position was effectively synthesized in 19 steps for the longest linear sequence and in a ca. 1% overall yield from 1,2-distearoyl-sn-glycerol and D-glucose. This compound was designed as a biosynthetic precursor of glycosylphosphatidylinositol (GPI) anchors. Its azide would enable further modification to introduce other molecular tags by a biocompatible click reaction. Therefore, it can be a useful probe for metabolic engineering of cell surface GPI anchors and GPI-anchored proteins.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.