Caliope Mendarte-Alquisira, Alejandro Alarcón, Ronald Ferrera-Cerrato
{"title":"加入拟除虫菊酯类杀虫剂的培养基中毛霉菌株的生长、耐受性和酶活性","authors":"Caliope Mendarte-Alquisira, Alejandro Alarcón, Ronald Ferrera-Cerrato","doi":"10.1016/j.ram.2023.06.004","DOIUrl":null,"url":null,"abstract":"<div><p>The application of pyrethroids and carbamates represents an environmental risk and may exert adverse effects on beneficial microorganisms such as <em>Trichoderma</em>, which contribute to the biocontrol of several fungal phytopathogens. This research evaluated the tolerance of several strains of <em>Trichoderma</em> to a selected culture medium contaminated with a commercial insecticide (H24®) composed of pyrethroids, permethrin and prallethrin, and carbamate propoxur, and determined the influence of this insecticide on the release of enzymes such as chitinases, peroxidases, and endoglucanases by a consortium of selected <em>Trichoderma</em> strains grown in liquid culture medium. Four out of 10 <em>Trichoderma</em> strains showed tolerance to 200<!--> <!-->ppm (∼48.3% of growth) of the commercial insecticide after 96<!--> <!-->h of exposure to a contaminated solid medium. After eight days of growth in liquid culture, the insecticide enhanced extracellular protein content and peroxidase activities in the <em>Trichoderma</em> consortium but decreased both chitinase and glucanase activities. These fungal responses should be considered when implementing strategies that combine alternative pesticides and fungal biocontrollers for managing fungal phytopathogens.</p></div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0325754123000597/pdfft?md5=4feb8875011e336f3a3fbce993f62818&pid=1-s2.0-S0325754123000597-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Growth, tolerance, and enzyme activities of Trichoderma strains in culture media added with a pyrethroids-based insecticide\",\"authors\":\"Caliope Mendarte-Alquisira, Alejandro Alarcón, Ronald Ferrera-Cerrato\",\"doi\":\"10.1016/j.ram.2023.06.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The application of pyrethroids and carbamates represents an environmental risk and may exert adverse effects on beneficial microorganisms such as <em>Trichoderma</em>, which contribute to the biocontrol of several fungal phytopathogens. This research evaluated the tolerance of several strains of <em>Trichoderma</em> to a selected culture medium contaminated with a commercial insecticide (H24®) composed of pyrethroids, permethrin and prallethrin, and carbamate propoxur, and determined the influence of this insecticide on the release of enzymes such as chitinases, peroxidases, and endoglucanases by a consortium of selected <em>Trichoderma</em> strains grown in liquid culture medium. Four out of 10 <em>Trichoderma</em> strains showed tolerance to 200<!--> <!-->ppm (∼48.3% of growth) of the commercial insecticide after 96<!--> <!-->h of exposure to a contaminated solid medium. After eight days of growth in liquid culture, the insecticide enhanced extracellular protein content and peroxidase activities in the <em>Trichoderma</em> consortium but decreased both chitinase and glucanase activities. These fungal responses should be considered when implementing strategies that combine alternative pesticides and fungal biocontrollers for managing fungal phytopathogens.</p></div>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0325754123000597/pdfft?md5=4feb8875011e336f3a3fbce993f62818&pid=1-s2.0-S0325754123000597-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0325754123000597\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0325754123000597","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Growth, tolerance, and enzyme activities of Trichoderma strains in culture media added with a pyrethroids-based insecticide
The application of pyrethroids and carbamates represents an environmental risk and may exert adverse effects on beneficial microorganisms such as Trichoderma, which contribute to the biocontrol of several fungal phytopathogens. This research evaluated the tolerance of several strains of Trichoderma to a selected culture medium contaminated with a commercial insecticide (H24®) composed of pyrethroids, permethrin and prallethrin, and carbamate propoxur, and determined the influence of this insecticide on the release of enzymes such as chitinases, peroxidases, and endoglucanases by a consortium of selected Trichoderma strains grown in liquid culture medium. Four out of 10 Trichoderma strains showed tolerance to 200 ppm (∼48.3% of growth) of the commercial insecticide after 96 h of exposure to a contaminated solid medium. After eight days of growth in liquid culture, the insecticide enhanced extracellular protein content and peroxidase activities in the Trichoderma consortium but decreased both chitinase and glucanase activities. These fungal responses should be considered when implementing strategies that combine alternative pesticides and fungal biocontrollers for managing fungal phytopathogens.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
