一种靶向传染性胃肠炎病毒刺突蛋白c端结构域的单克隆抗体。

Q3 Medicine

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2022-12-01 DOI:10.1089/mab.2022.0030

Na Liu, Yaoming Li

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引用次数: 0

摘要

猪传染性胃肠炎病毒(TGEV)刺突蛋白c端结构域(CTD)的结构和功能在很大程度上仍然未知，因此特异性单克隆抗体(MAb)使我们能够充分了解该结构域。本研究通过间接荧光实验、Western blotting和荧光激活细胞分选仪的实验结果证实，我们开发了一种抗TGEV刺突蛋白CTD的小鼠单抗。进一步研究表明，该单抗能够特异性识别TGEV刺突蛋白CTD衍生的12个残基肽(FKNVSDGVIYSV)。该单抗可用于阐明TGEV尖峰CTD在病毒附着和进入中的潜在功能，值得进一步深入研究。

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A Monoclonal Antibody Targeting C-Terminal Domain of Transmissible Gastroenteritis Virus Spike Protein.

The structure and function of the C-terminus domain (CTD) of porcine transmissible gastroenteritis virus (TGEV) spike protein remain largely unknown, thereby a specific monoclonal antibody (MAb) allows us to fully understand this domain. In this study, we developed a murine MAb against CTD of TGEV spike protein, as evidenced by the results of indirect fluorescent assay, Western blotting, and fluorescence-activated cell sorter. Further study showed that the MAb is able to exclusively recognize a 12-residue peptide (FKNVSDGVIYSV) derived from CTD of TGEV spike protein. This MAb can be used to elucidate the potential function of CTD of TGEV spike in virus attachment and entry, and warrants further intensive investigation.

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来源期刊

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Medicine-Immunology and Allergy

CiteScore

4.80

自引率

0.00%

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