道场泥鳅5S核糖体DNA核苷酸序列及染色体定位。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Kiko Shibata, Masamichi Kuroda, Etsuro Yamaha, Katsutoshi Arai, Takafumi Fujimoto
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引用次数: 1

摘要

泥鳅(Misgurnus anguillicaudatus)有2个遗传差异群体:A和b。尽管大多数野生型二倍体有性生殖,但在某些地区,无性系二倍体(无性系泥鳅)是雌性生殖。克隆泥鳅通过减数分裂前内膜分裂产生未减少的等基因卵,这些二倍体卵在同域野生型二倍体精子的激活下发育成雌体。这些克隆泥鳅可能是由两个不同祖先之间的杂交事件产生的。这两组之间的基因组差异尚未完全阐明。因此,需要新的遗传和细胞遗传学标记来区分这两组。在此,我们比较了5S rDNA区域,开发了不同dojo泥鳅类群的标记。5S rDNA的非转录序列(NTS)具有高度多态性和群体特异性。克隆泥鳅在2个不同类群的枝上均发现了nts。相比之下,我们在5S rRNA基因的编码区未发现任何组特异性序列。序列位于A组和B组克隆泥鳅最大的亚着丝中心染色体短臂的着丝粒附近。因此,道场泥鳅的5S rDNA在染色体位置上是保守的。而NTS区域的序列在道场泥鳅中具有群体特异性,在克隆泥鳅中这两个群体的序列都是保守的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nucleotide Sequence and Chromosome Mapping of 5S Ribosomal DNA from the Dojo Loach, Misgurnus anguillicaudatus.

There are 2 genetically divergent groups in the dojo loach Misgurnus anguillicaudatus: A and B. Although most wild-type diploids reproduce sexually, clonal diploids (clonal loach) reproduce gynogenetically in certain areas. Clonal loaches produce unreduced isogenic eggs by premeiotic endomitosis, and such diploid eggs develop gynogenetically following activation by the sperm of sympatric wild-type diploids. These clonal loaches have presumably arisen from past hybridization events between 2 different ancestors. The genomic differences between these 2 groups have not been completely elucidated. Thus, new genetic and cytogenetic markers are required to distinguish between these 2 groups. Here, we compared the 5S rDNA region to develop markers for the identification of different dojo loach groups. The nontranscribed sequence (NTS) of the 5S rDNA was highly polymorphic and group-specific. NTSs were found in clades of 2 different groups in clonal loaches. In contrast, we did not find any group-specific sequences in the coding region of the 5S rRNA gene. Sequences were located near the centromere of the short arm of the largest submetacentric chromosomes in groups A and B and clonal loaches. Thus, the 5S rDNA of the dojo loach is conserved at the chromosomal location. Whereas, the sequences of the NTS regions evolved group-specifically in the dojo loach, with the sequences of both groups being conserved in clonal loaches.

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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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