2型糖尿病和非糖尿病患者颈动脉粥样硬化斑块中脂质介质的分析

IF 3
Louise Ménégaut , Aline Laubriet , Valentin Crespy , Maxime Nguyen , Jean-Michel Petit , Georges Tarris , Thomas Pilot , Alexis Varin , Hélène Choubley , Victoria Bergas , Jean-Paul Pais de Barros , Charles Thomas , Eric Steinmetz , David Masson
{"title":"2型糖尿病和非糖尿病患者颈动脉粥样硬化斑块中脂质介质的分析","authors":"Louise Ménégaut ,&nbsp;Aline Laubriet ,&nbsp;Valentin Crespy ,&nbsp;Maxime Nguyen ,&nbsp;Jean-Michel Petit ,&nbsp;Georges Tarris ,&nbsp;Thomas Pilot ,&nbsp;Alexis Varin ,&nbsp;Hélène Choubley ,&nbsp;Victoria Bergas ,&nbsp;Jean-Paul Pais de Barros ,&nbsp;Charles Thomas ,&nbsp;Eric Steinmetz ,&nbsp;David Masson","doi":"10.1016/j.plefa.2022.102477","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aims</h3><p>Diabetes is associated with an accelerated development of atherosclerosis. Specific mechanisms related to diabetes and hyperglycemia may play a role in this process. In particular, alterations of arachidonic acid (AA) metabolism have been reported. Our main goal was to investigate for differences in the concentration of LTB4 and RvD1 as well as selected cyclooxygenase-derived mediators in carotid plaques from diabetic and non-diabetic patients. We also aimed to analyze the relationship between omega 6 and omega 3 Poly-Unsaturated Fatty acids (PUFAs) content in the plaques and the concentrations of these lipid mediators.</p></div><div><h3>Methods</h3><p>29 type 2 diabetic patients and 30 control patients admitted for surgical treatment of carotid stenosis were enrolled in the present study. Carotid plaques were harvested for in-depth lipidomic profiling.</p></div><div><h3>Results</h3><p>No differences for LTB4 or other lipid mediators were observed between diabetic and non-diabetic patients. RvD1 levels were below the threshold of quantification in most of the samples. A significant correlation was found between LTB4 and 5(<em>S</em>)-HETE levels. Omega 3 enrichment was not significantly different between control and diabetic plaques. There was a negative correlation between DHA/AA ratio and the level of 5(<em>S</em>)-HETE while there was a positive association with TXB2 and PGD2 concentrations.</p></div><div><h3>Conclusion-perspectives</h3><p>Our results does not support the hypothesis of a specific involvement of LTB4 or COX-derived mediators in diabetic atherosclerosis. The relationship between DHA enrichment and the concentrations of specific inflammatory mediators within the plaque is of interest and will need to be confirmed in larger studies.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Profiling of lipid mediators in atherosclerotic carotid plaques from type 2 diabetic and non-diabetic patients.\",\"authors\":\"Louise Ménégaut ,&nbsp;Aline Laubriet ,&nbsp;Valentin Crespy ,&nbsp;Maxime Nguyen ,&nbsp;Jean-Michel Petit ,&nbsp;Georges Tarris ,&nbsp;Thomas Pilot ,&nbsp;Alexis Varin ,&nbsp;Hélène Choubley ,&nbsp;Victoria Bergas ,&nbsp;Jean-Paul Pais de Barros ,&nbsp;Charles Thomas ,&nbsp;Eric Steinmetz ,&nbsp;David Masson\",\"doi\":\"10.1016/j.plefa.2022.102477\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and aims</h3><p>Diabetes is associated with an accelerated development of atherosclerosis. Specific mechanisms related to diabetes and hyperglycemia may play a role in this process. In particular, alterations of arachidonic acid (AA) metabolism have been reported. Our main goal was to investigate for differences in the concentration of LTB4 and RvD1 as well as selected cyclooxygenase-derived mediators in carotid plaques from diabetic and non-diabetic patients. We also aimed to analyze the relationship between omega 6 and omega 3 Poly-Unsaturated Fatty acids (PUFAs) content in the plaques and the concentrations of these lipid mediators.</p></div><div><h3>Methods</h3><p>29 type 2 diabetic patients and 30 control patients admitted for surgical treatment of carotid stenosis were enrolled in the present study. Carotid plaques were harvested for in-depth lipidomic profiling.</p></div><div><h3>Results</h3><p>No differences for LTB4 or other lipid mediators were observed between diabetic and non-diabetic patients. RvD1 levels were below the threshold of quantification in most of the samples. A significant correlation was found between LTB4 and 5(<em>S</em>)-HETE levels. Omega 3 enrichment was not significantly different between control and diabetic plaques. There was a negative correlation between DHA/AA ratio and the level of 5(<em>S</em>)-HETE while there was a positive association with TXB2 and PGD2 concentrations.</p></div><div><h3>Conclusion-perspectives</h3><p>Our results does not support the hypothesis of a specific involvement of LTB4 or COX-derived mediators in diabetic atherosclerosis. The relationship between DHA enrichment and the concentrations of specific inflammatory mediators within the plaque is of interest and will need to be confirmed in larger studies.</p></div>\",\"PeriodicalId\":94179,\"journal\":{\"name\":\"Prostaglandins, leukotrienes, and essential fatty acids\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins, leukotrienes, and essential fatty acids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0952327822000898\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and essential fatty acids","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0952327822000898","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

背景和目的糖尿病与动脉粥样硬化的加速发展有关。与糖尿病和高血糖相关的特定机制可能在这一过程中发挥作用。特别是花生四烯酸(AA)代谢的改变已被报道。我们的主要目的是研究糖尿病和非糖尿病患者颈动脉斑块中LTB4和RvD1浓度以及所选环氧化酶衍生介质的差异。我们还旨在分析斑块中omega 6和omega 3多不饱和脂肪酸(PUFAs)含量与这些脂质介质浓度之间的关系。方法选择手术治疗颈动脉狭窄的2型糖尿病患者29例,对照组30例。采集颈动脉斑块进行深度脂质组学分析。结果糖尿病患者与非糖尿病患者LTB4及其他脂质介质无差异。大多数样品的RvD1水平低于定量阈值。LTB4与5(S)-HETE水平存在显著相关性。Omega - 3富集在对照组和糖尿病斑块之间没有显著差异。DHA/AA比值与5(S)-HETE水平呈负相关,与TXB2、PGD2浓度呈正相关。我们的研究结果不支持LTB4或cox衍生介质特异性参与糖尿病动脉粥样硬化的假设。DHA富集与斑块内特定炎症介质浓度之间的关系令人感兴趣,需要在更大规模的研究中得到证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Profiling of lipid mediators in atherosclerotic carotid plaques from type 2 diabetic and non-diabetic patients.

Background and aims

Diabetes is associated with an accelerated development of atherosclerosis. Specific mechanisms related to diabetes and hyperglycemia may play a role in this process. In particular, alterations of arachidonic acid (AA) metabolism have been reported. Our main goal was to investigate for differences in the concentration of LTB4 and RvD1 as well as selected cyclooxygenase-derived mediators in carotid plaques from diabetic and non-diabetic patients. We also aimed to analyze the relationship between omega 6 and omega 3 Poly-Unsaturated Fatty acids (PUFAs) content in the plaques and the concentrations of these lipid mediators.

Methods

29 type 2 diabetic patients and 30 control patients admitted for surgical treatment of carotid stenosis were enrolled in the present study. Carotid plaques were harvested for in-depth lipidomic profiling.

Results

No differences for LTB4 or other lipid mediators were observed between diabetic and non-diabetic patients. RvD1 levels were below the threshold of quantification in most of the samples. A significant correlation was found between LTB4 and 5(S)-HETE levels. Omega 3 enrichment was not significantly different between control and diabetic plaques. There was a negative correlation between DHA/AA ratio and the level of 5(S)-HETE while there was a positive association with TXB2 and PGD2 concentrations.

Conclusion-perspectives

Our results does not support the hypothesis of a specific involvement of LTB4 or COX-derived mediators in diabetic atherosclerosis. The relationship between DHA enrichment and the concentrations of specific inflammatory mediators within the plaque is of interest and will need to be confirmed in larger studies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
发文量
0
审稿时长
64 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信