减少亚油酸和增加二十碳五烯酸和二十二碳六烯酸对慢性单关节炎模型大鼠的生化和行为影响

IF 3
Anthony F. Domenichiello , Breanne C. Wilhite , Pranavi Nara , Mark H. Pitcher , Gregory S. Keyes , Andrew J. Mannes , M. Catherine Bushnell , Christopher E. Ramsden
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引用次数: 1

摘要

临床研究表明,在饮食中减少亚油酸(LA)而增加二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)可引起头痛患者的镇痛作用。我们利用大鼠慢性单关节炎模型来确定这些镇痛作用是否可以在大鼠中复制,并进一步探索潜在的镇痛机制。我们给8只大鼠喂食对照饮食(脂肪酸水平与标准美国饮食相似),8只大鼠喂食添加EPA和DHA的低LA饮食(H3L6饮食),10周后,进行单侧关节内注射Complete's Freund佐剂(CFA)。我们评估了注射CFA前、注射后4天和20天的热和机械敏感性以及后爪负重。注射CFA后28天,对大鼠实施安乐死并收集组织。与对照组相比,H3L6饮食喂养的大鼠在后爪和血浆中含有更高浓度的EPA和DHA,以及由这些脂肪酸产生的氧化脂质浓度更高。与对照组相比,H3L6大鼠血浆和后爪中的LA和氧化LA代谢物含量较低。饮食不影响热或机械敏感性,也不影响后爪负重。综上所述,H3L6饮食引起了大鼠的生化变化,但对慢性单关节炎模型中疼痛相关的行为措施没有影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biochemical and behavioral effects of decreasing dietary linoleic acid and increasing eicosapentaenoic acid and docosahexaenoic acid in a rat chronic monoarthrits model

Clinical studies have demonstrated that decreasing linoleic acid (LA) while increasing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in diets evokes an analgesic effect in headache sufferers. We utilized a rat chronic monoarthritis model to determine if these analgesic effects can be reproduced in rats and to and further probe potential analgesic mechanisms. We fed 8 rats a control diet (with fatty acid levels similar to standard US diets) and 8 rats a low LA diet with added EPA and DHA (H3L6 diet) and after 10 weeks, performed a unilateral intraarticular injection of Complete's Freund Adjuvant (CFA). We evaluated thermal and mechanical sensitivity as well as hind paw weight bearing prior to and at 4 and 20 days post CFA injection. At 28 days post CFA injection rats were euthanized and tissue collected. H3L6 diet fed rats had higher concentrations of EPA and DHA, as well as higher concentrations of oxidized lipids derived from these fatty acids, in hind paw and plasma, compared to control fed rats. LA and oxidized LA metabolites were lower in the plasma and hind paw of H3L6 compared to control fed rats. Diet did not affect thermal or mechanical sensitivity, nor did it affect hind paw weight bearing. In conclusion, the H3L6 diet evoked biochemical changes in rats but did not impact pain related behavioral measures in this chronic monoarthritis model.

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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
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审稿时长
64 days
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