Gabriela Leite de Souza, Thamara Eduarda Alves Magalhães, Gabrielle Alves Nunes Freitas, Nelly Xiomara Alvarado Lemus, Gabriella Lopes de Rezende Barbosa, Anielle Christine Almeida Silva, Camilla Christian Gomes Moura
{"title":"掺钙氧化锌纳米晶体作为一种创新的龋内药物：一项试验研究。","authors":"Gabriela Leite de Souza, Thamara Eduarda Alves Magalhães, Gabrielle Alves Nunes Freitas, Nelly Xiomara Alvarado Lemus, Gabriella Lopes de Rezende Barbosa, Anielle Christine Almeida Silva, Camilla Christian Gomes Moura","doi":"10.5395/rde.2022.47.e38","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the cytotoxicity, radiopacity, pH, and dentinal tubule penetration of a paste of 1.0% calcium-doped zinc oxide nanocrystals (ZnO:1.0Ca) combined with propylene glycol (PRG) or polyethylene glycol and propylene glycol (PEG-PRG).</p><p><strong>Materials and methods: </strong>The pastes were prepared by mixing calcium hydroxide [Ca(OH)<sub>2</sub>] or ZnO:1.0Ca with PRG or a PEG-PRG mixture. The pH was evaluated after 24 and 96 hours of storage in deionized water. Digital radiographs were acquired for radiopacity analysis and bubble counting of each material. The materials were labeled with 0.1% fluorescein and applied to root canals, and images of their dentinal tubule penetration were obtained using confocal laser scanning microscopy. RAW264.7 macrophages were placed in different dilutions of culture media previously exposed to the materials for 24 and 96 hours and tested for cell viability using the MTT assay. Analysis of variance and the Tukey test (<i>α</i> = 0.05) were performed.</p><p><strong>Results: </strong>ZnO:1.0Ca materials showed lower viability at 1:1 and 1:2 dilutions than Ca(OH)<sub>2</sub> materials (<i>p</i> < 0.0001). Ca(OH)<sub>2</sub> had higher pH values than ZnO:1.0Ca at 24 and 96 hours, regardless of the vehicle (<i>p</i> < 0.05). ZnO:1.0Ca pastes showed higher radiopacity than Ca(OH)<sub>2</sub> pastes (<i>p</i> < 0.01). No between-material differences were found in bubble counting (<i>p</i> = 0.0902). The ZnO:1.0Ca pastes had a greater penetration depth than Ca(OH)<sub>2</sub> in the apical third (<i>p</i> < 0.0001).</p><p><strong>Conclusions: </strong>ZnO:1.0Ca medicaments presented higher penetrability, cell viability, and radiopacity than Ca(OH)<sub>2</sub>. Higher values of cell viability and pH were present in Ca(OH)<sub>2</sub> than in ZnO:1.0Ca.</p>","PeriodicalId":21102,"journal":{"name":"Restorative Dentistry & Endodontics","volume":"47 4","pages":"e38"},"PeriodicalIF":0.0000,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/63/rde-47-e38.PMC9715372.pdf","citationCount":"0","resultStr":"{\"title\":\"Calcium-doped zinc oxide nanocrystals as an innovative intracanal medicament: a pilot study.\",\"authors\":\"Gabriela Leite de Souza, Thamara Eduarda Alves Magalhães, Gabrielle Alves Nunes Freitas, Nelly Xiomara Alvarado Lemus, Gabriella Lopes de Rezende Barbosa, Anielle Christine Almeida Silva, Camilla Christian Gomes Moura\",\"doi\":\"10.5395/rde.2022.47.e38\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>This study investigated the cytotoxicity, radiopacity, pH, and dentinal tubule penetration of a paste of 1.0% calcium-doped zinc oxide nanocrystals (ZnO:1.0Ca) combined with propylene glycol (PRG) or polyethylene glycol and propylene glycol (PEG-PRG).</p><p><strong>Materials and methods: </strong>The pastes were prepared by mixing calcium hydroxide [Ca(OH)<sub>2</sub>] or ZnO:1.0Ca with PRG or a PEG-PRG mixture. The pH was evaluated after 24 and 96 hours of storage in deionized water. Digital radiographs were acquired for radiopacity analysis and bubble counting of each material. The materials were labeled with 0.1% fluorescein and applied to root canals, and images of their dentinal tubule penetration were obtained using confocal laser scanning microscopy. RAW264.7 macrophages were placed in different dilutions of culture media previously exposed to the materials for 24 and 96 hours and tested for cell viability using the MTT assay. Analysis of variance and the Tukey test (<i>α</i> = 0.05) were performed.</p><p><strong>Results: </strong>ZnO:1.0Ca materials showed lower viability at 1:1 and 1:2 dilutions than Ca(OH)<sub>2</sub> materials (<i>p</i> < 0.0001). Ca(OH)<sub>2</sub> had higher pH values than ZnO:1.0Ca at 24 and 96 hours, regardless of the vehicle (<i>p</i> < 0.05). ZnO:1.0Ca pastes showed higher radiopacity than Ca(OH)<sub>2</sub> pastes (<i>p</i> < 0.01). No between-material differences were found in bubble counting (<i>p</i> = 0.0902). The ZnO:1.0Ca pastes had a greater penetration depth than Ca(OH)<sub>2</sub> in the apical third (<i>p</i> < 0.0001).</p><p><strong>Conclusions: </strong>ZnO:1.0Ca medicaments presented higher penetrability, cell viability, and radiopacity than Ca(OH)<sub>2</sub>. Higher values of cell viability and pH were present in Ca(OH)<sub>2</sub> than in ZnO:1.0Ca.</p>\",\"PeriodicalId\":21102,\"journal\":{\"name\":\"Restorative Dentistry & Endodontics\",\"volume\":\"47 4\",\"pages\":\"e38\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/63/rde-47-e38.PMC9715372.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Restorative Dentistry & Endodontics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5395/rde.2022.47.e38\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/11/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Restorative Dentistry & Endodontics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5395/rde.2022.47.e38","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/11/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Calcium-doped zinc oxide nanocrystals as an innovative intracanal medicament: a pilot study.
Objectives: This study investigated the cytotoxicity, radiopacity, pH, and dentinal tubule penetration of a paste of 1.0% calcium-doped zinc oxide nanocrystals (ZnO:1.0Ca) combined with propylene glycol (PRG) or polyethylene glycol and propylene glycol (PEG-PRG).
Materials and methods: The pastes were prepared by mixing calcium hydroxide [Ca(OH)2] or ZnO:1.0Ca with PRG or a PEG-PRG mixture. The pH was evaluated after 24 and 96 hours of storage in deionized water. Digital radiographs were acquired for radiopacity analysis and bubble counting of each material. The materials were labeled with 0.1% fluorescein and applied to root canals, and images of their dentinal tubule penetration were obtained using confocal laser scanning microscopy. RAW264.7 macrophages were placed in different dilutions of culture media previously exposed to the materials for 24 and 96 hours and tested for cell viability using the MTT assay. Analysis of variance and the Tukey test (α = 0.05) were performed.
Results: ZnO:1.0Ca materials showed lower viability at 1:1 and 1:2 dilutions than Ca(OH)2 materials (p < 0.0001). Ca(OH)2 had higher pH values than ZnO:1.0Ca at 24 and 96 hours, regardless of the vehicle (p < 0.05). ZnO:1.0Ca pastes showed higher radiopacity than Ca(OH)2 pastes (p < 0.01). No between-material differences were found in bubble counting (p = 0.0902). The ZnO:1.0Ca pastes had a greater penetration depth than Ca(OH)2 in the apical third (p < 0.0001).
Conclusions: ZnO:1.0Ca medicaments presented higher penetrability, cell viability, and radiopacity than Ca(OH)2. Higher values of cell viability and pH were present in Ca(OH)2 than in ZnO:1.0Ca.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
