线粒体代谢示踪剂F-AraG在健康志愿者中的生物分布[18F]。

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Jelena Levi, Heying Duan, Shahriar Yaghoubi, Juliet Packiasamy, Lyna Huynh, Tina Lam, Faiq Shaikh, Deepak Behera, Hong Song, Joseph Blecha, Salma Jivan, Youngho Seo, Henry F VanBrocklin
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引用次数: 5

摘要

目的:[18F]F-AraG是一种放射性标记的核苷类似物,对活化的T细胞具有相对特异性。本研究的目的是研究[18F]F-AraG在健康志愿者中的生物分布,并评估其初步安全性和辐射剂量学。方法:选取年龄在24 ~ 60岁之间的健康受试者6名,男女各3名。每位受试者在连续四次PET/MR全身扫描之前,接受静脉注射[18F]F-AraG(剂量范围:244.2-329.3 MBq)。定期采集血液样本,监测示踪剂使用前后的生命体征。绘制多个器官的感兴趣区域,计算每个器官的时间-活动曲线下的面积,并用于推导时间积分活动系数(TIAC)。输入TIACs,使用OLINDA计算吸收剂量和有效剂量。结果:PET/MR检查耐受良好,研究参与者未注意到对[18F]F-AraG的不良反应。生物分布总体上反映了参与[18F]F-AraG细胞积累的酶、线粒体激酶dGK和SAMHD1的表达和活性谱。肾脏和肝脏对示踪剂的摄取最高,而脑、肺、骨髓和肌肉对示踪剂的摄取较低。[18F]F-AraG的估计有效剂量为0.0162 mSv/MBq(女性为0.0167 mSv/MBq,男性为0.0157 mSv/MBq)。结论:[18F]F-AraG在健康志愿者体内的生物分布与其与线粒体代谢的关系一致。PET/MR [18F]F-AraG成像耐受性良好,其辐射剂量谱与其他常用的[18F]标记示踪剂相似。[18F]F-AraG与线粒体生物发生的联系和良好的生物分布特性使其成为一种有吸引力的示踪剂,具有多种潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biodistribution of a Mitochondrial Metabolic Tracer, [<sup>18</sup>F]F-AraG, in Healthy Volunteers.

Biodistribution of a Mitochondrial Metabolic Tracer, [<sup>18</sup>F]F-AraG, in Healthy Volunteers.

Biodistribution of a Mitochondrial Metabolic Tracer, [<sup>18</sup>F]F-AraG, in Healthy Volunteers.

Biodistribution of a Mitochondrial Metabolic Tracer, [18F]F-AraG, in Healthy Volunteers.

Purpose: [18F]F-AraG is a radiolabeled nucleoside analog that shows relative specificity for activated T cells. The aim of this study was to investigate the biodistribution of [18F]F-AraG in healthy volunteers and assess the preliminary safety and radiation dosimetry.

Methods: Six healthy subjects (three female and three male) between the ages of 24 and 60 participated in the study. Each subject received a bolus venous injection of [18F]F-AraG (dose range: 244.2-329.3 MBq) prior to four consecutive PET/MR whole-body scans. Blood samples were collected at regular intervals and vital signs monitored before and after tracer administration. Regions of interest were delineated for multiple organs, and the area under the time-activity curves was calculated for each organ and used to derive time-integrated activity coefficient (TIAC). TIACs were input for absorbed dose and effective dose calculations using OLINDA.

Results: PET/MR examination was well tolerated, and no adverse effects to the administration of [18F]F-AraG were noted by the study participants. The biodistribution was generally reflective of the expression and activity profiles of the enzymes involved in [18F]F-AraG's cellular accumulation, mitochondrial kinase dGK, and SAMHD1. The highest uptake was observed in the kidneys and liver, while the brain, lung, bone marrow, and muscle showed low tracer uptake. The estimated effective dose for [18F]F-AraG was 0.0162 mSv/MBq (0.0167 mSv/MBq for females and 0.0157 mSv/MBq for males).

Conclusion: Biodistribution of [18F]F-AraG in healthy volunteers was consistent with its association with mitochondrial metabolism. PET/MR [18F]F-AraG imaging was well tolerated, with a radiation dosimetry profile similar to other commonly used [18F]-labeled tracers. [18F]F-AraG's connection with mitochondrial biogenesis and favorable biodistribution characteristics make it an attractive tracer with a variety of potential applications.

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来源期刊
Molecular Imaging
Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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