MiR-320a 通过靶向 BCAT1 在嗜体细胞垂体神经内分泌肿瘤中发挥抑癌作用

IF 3.2 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Neuroendocrinology Pub Date : 2024-01-01 Epub Date: 2023-08-17 DOI:10.1159/000533549
Sida Zhao, Bin Li, Hua Gao, Yazhuo Zhang
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引用次数: 0

摘要

简介据报道,miR-320a的异常参与了多种癌症的发生。在我们之前的研究中,我们发现体细胞垂体神经内分泌肿瘤(PitNET)患者的循环 miR-320a 低表达;然而,miR-320a 在体细胞垂体神经内分泌肿瘤增殖中的作用仍不清楚:方法:采用细胞活力和集落形成试验检测 miR-320a 和 BCAT1 对 GH3 细胞的影响。TargetScan 用于鉴定 miR-320a 的靶基因。采用双荧光素酶报告基因检测法探讨 miR-320a 和 BCAT1 之间的关系。对体细胞瘤 PitNET 和健康对照组进行了转录组和蛋白质组分析。结果显示:miR-320a模拟物抑制细胞增殖,而miR-320a抑制剂则促进GH3细胞的增殖。利用维恩图进行的重叠分析表明,与健康对照组相比,BCAT1是miR-320a在嗜体细胞PitNET中表达过高的唯一靶基因,这一点在芯片和蛋白质组学结果中均有体现。双荧光素酶报告基因测定显示,miR-320a可能与BCAT1-3'UTR结合。转染miR-320a模拟物可下调GH3细胞中BCAT1的表达,而miR-320a抑制物则可上调BCAT1的表达。干扰 BCAT1 在 GH3 细胞中的表达会降低细胞的增殖和生长。泛癌分析表明,BCAT1的高表达通常预示着不良预后:我们的研究结果表明,miR-320a 可作为肿瘤抑制因子发挥作用,而 BCAT1 可促进肿瘤进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MiR-320a Acts as a Tumor Suppressor in Somatotroph Pituitary Neuroendocrine Tumors by Targeting BCAT1.

Introduction: Aberrant miR-320a has been reported to be involved in the tumorigenesis of several cancers. In our previous study, we identified the low expression of circulating miR-320a in patients with somatotroph pituitary neuroendocrine tumor (PitNET); however, the role of miR-320a in somatotroph PitNET proliferation is still unclear.

Methods: Cell viability and colony formation assays were used to detect the effect of miR-320a and BCAT1 on GH3 cells. TargetScan was used to identify the target genes of miR-320a. Dual-luciferase reporter gene assay was used to explore the relation between miR-320a and BCAT1. Transcriptome and proteome analyses were performed between somatotroph PitNETs and healthy controls. The expression level of miR-320a in somatotroph PitNETs were detected by RT-qPCR and Western blot.

Results: miR-320a mimics inhibit cell proliferation, while miR-320a inhibitors promote cell proliferation in GH3 cells. An overlap analysis using a Venn diagram revealed that BCAT1 is the only target gene of miR-320a overexpressed in somatotroph PitNETs compared to healthy controls, as revealed by both microarray and proteomics results. A dual-luciferase reporter gene assay showed that miR-320a may bind to the BCAT1-3'UTR. The transfection of miR-320a mimics downregulated the expression and miR-320a inhibitors and upregulated the expression of BCAT1 in GH3 cells. The interference of BCAT1 expression in GH3 cells downregulated cell proliferation and growth. Pan-cancer analyses demonstrated that high BCAT1 expression often indicates a poor prognosis.

Conclusion: Our findings illustrate that miR-320a may function as a tumor suppressor and BCAT1 may promote tumor progression. miR-320a may inhibit the growth of somatotroph PitNETs by targeting BCAT1.

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来源期刊
Neuroendocrinology
Neuroendocrinology 医学-内分泌学与代谢
CiteScore
8.30
自引率
2.40%
发文量
50
审稿时长
6-12 weeks
期刊介绍: ''Neuroendocrinology'' publishes papers reporting original research in basic and clinical neuroendocrinology. The journal explores the complex interactions between neuronal networks and endocrine glands (in some instances also immunecells) in both central and peripheral nervous systems. Original contributions cover all aspects of the field, from molecular and cellular neuroendocrinology, physiology, pharmacology, and the neuroanatomy of neuroendocrine systems to neuroendocrine correlates of behaviour, clinical neuroendocrinology and neuroendocrine cancers. Readers also benefit from reviews by noted experts, which highlight especially active areas of current research, and special focus editions of topical interest.
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