商用DURAClone面板,用于扩展南非学术流式细胞术实验室的多色免疫表型面板。

IF 1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Leanne Swart, Melanie Pretorius, Denise Lawrie, Deborah K Glencross
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引用次数: 0

摘要

背景:商用多色固定免疫表型板可以改善流式细胞术诊断免疫表型库。目的:本研究验证了市售的、标准化的Beckman Coulter冻干DURAClone RE检测板能够区分特定的淋巴细胞亚型。方法:2020年4月至8月,我们比较了南非约翰内斯堡Charlotte Maxeke约翰内斯堡学术医院DURAClone急性B型白血病(ALB)、慢性B型白血病(CLB)和浆细胞(PC)检测结果与预测二线检测结果的诊断能力。建立了内部二线免疫表型(谓词法)与DURAClone的临床诊断一致性。ALB组检测前体b细胞急性淋巴细胞白血病(n = 11)或正常骨髓血球(n = 9);CLB组检测成熟b细胞淋巴增生性疾病(B-LPD)的血淋巴亚型(n = 20),而PC组检测浆细胞增生(PCD) (n = 17)。流式细胞仪设置和数据解释,以区分正常和异常的免疫表型按照制造商的说明。结果:在B-ALL(附加CD56)、成熟B-LPD(附加CD81、r -1、CD79b和CD43)和PCD的二线诊断调查中,predicate与试验组的临床诊断一致性为100%。结论:DURAClone CLB超过了预期的二线性能,提供了成熟B-LPD亚型的扩展二线诊断识别以及CD5+ B-LPD与其他非CD5+(或CD5-) B-LPD的区分;同样,PC面板使PCD的发现成为可能。虽然与现有的谓词调查相比,ALB检测没有提供额外的诊断优势,但CD58确实提供了从B-ALL中区分血精的额外信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Commercial DURAClone panels for extending the repertoire of multicolour immunophenotypic panels in an academic flow cytometry laboratory in South Africa.

Commercial DURAClone panels for extending the repertoire of multicolour immunophenotypic panels in an academic flow cytometry laboratory in South Africa.

Commercial DURAClone panels for extending the repertoire of multicolour immunophenotypic panels in an academic flow cytometry laboratory in South Africa.

Background: Commercial multicolour fixed immunophenotyping panels can improve flow cytometric diagnostic immunophenotyping repertoire.

Objective: This study validated the commercially available, standardised Beckman Coulter lyophilised DURAClone RE panels to discriminate specific haematolymphoid subtypes.

Methods: We compared the diagnostic capability of the DURAClone acute leukaemia B (ALB), chronic leukaemia B (CLB), and plasma cells (PC) panels to the predicate second-line panels in Charlotte Maxeke Johannesburg Academic Hospital, Johannesburg, South Africa, from April to August 2020. Clinical diagnostic concordance between the in-house second-line immunophenotyping (the predicate method) and DURAClone was established. The ALB panels tested for precursor B-cell acute lymphoblastic leukaemia (n = 11) or normal bone marrow haematogones (n = 9); CLB panels established haematolymphoid subtypes of mature B-cell lymphoproliferative disorders (B-LPD) (n = 20), while PC panels detected plasma cell dyscrasias (PCD) (n = 17). Flow cytometer setup and data interpretation to discriminate normal and aberrant immunophenotypes were per manufacturer's instructions.

Results: There was 100% clinical diagnostic concordance between the predicate and the test panels for second-line diagnostic investigation of B-ALL (with additional CD56), mature B-LPD (with additional discernment of CD81, ROR-1, CD79b and CD43) and PCD.

Conclusion: The DURAClone CLB exceeded the predicate second-line performance, offering extended second-line diagnostic discernment of mature B-LPD subtypes and discernment of CD5+ B-LPD from other non-CD5+ (or CD5-) B-LPD; likewise, the PC panels enabled discovery of PCD. While ALB testing offered no additional diagnostic advantage over existing predicate investigation, CD58 did offer additional information to discern haematogones from B-ALL.

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来源期刊
African Journal of Laboratory Medicine
African Journal of Laboratory Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
1.70
自引率
9.10%
发文量
53
审稿时长
12 weeks
期刊介绍: The African Journal of Laboratory Medicine, the official journal of ASLM, focuses on the role of the laboratory and its professionals in the clinical and public healthcare sectors,and is specifically based on an African frame of reference. Emphasis is on all aspects that promote and contribute to the laboratory medicine practices of Africa. This includes, amongst others: laboratories, biomedical scientists and clinicians, medical community, public health officials and policy makers, laboratory systems and policies (translation of laboratory knowledge, practices and technologies in clinical care), interfaces of laboratory with medical science, laboratory-based epidemiology, laboratory investigations, evidence-based effectiveness in real world (actual) settings.
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