鳉鱼早期胚胎发育过程中伽马射线照射引起的视网膜损伤后的再生。

IF 2.1 4区 医学 Q2 BIOLOGY
Takako Yasuda, Takuya Nakazawa, Kei Hirakawa, Ikumi Matsumoto, Kento Nagata, Shunta Mori, Kento Igarashi, Hiroshi Sagara, Shoji Oda, Hiroshi Mitani
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引用次数: 0

摘要

目的:斑马鱼是一种小型鱼类模型,其视网膜在损伤后终生都具有多能再生能力。与斑马鱼相比,鸟类和哺乳动物只有在胚胎时期才表现出这种再生能力,而且这种能力会随着年龄的增长而下降。青鳉是另一种在生物学研究中被广泛使用的小型鱼类模型,在受精后八天(dpf)的幼体中,视网膜内核层(INL)在损伤后无法再生。我们研究了青鳉胚胎早期视网膜损伤时的再生过程:方法:我们在青鳉胚胎3 dpf时使用10 Gy剂量的伽马射线照射来引发视网膜损伤,并对21 dpf前的胚胎进行组织病理学分析:结果:辐照一天后,在INL中观察到大量凋亡的神经元;但在睫状缘区和感光层中很少观察到这些神经元。直到照射两天后,受照射的视网膜上才出现大量凋亡细胞。这些细胞在照射四天后消失,但 INL 和神经节细胞层(GCL)之间的异常桥接结构在照射 11 天后仍然存在,神经层在照射 18 天后完全再生。伽马射线照射后,INL中纺锤形的Müller神经胶质细胞变得更圆,但并没有失去表达SOX2的能力:结论:辐照后3 dpf的青鳉胚胎视网膜可在辐照后18天(21 dpf)完全再生,尽管在所有辐照胚胎的视网膜中都短暂形成了连接INL和GCL的异常层结构。辐照四天后,青鳉胚胎 Müller 胶质数量减少,但与未受辐照的胚胎一样保持 SOX2 表达。这一发现与之前的报道形成了鲜明对比,之前的报道称 8 dpf 青鳉幼体不能完全再生受损视网膜的原因是 SOX2 表达的缺失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Retinal regeneration after injury induced by gamma-ray irradiation during early embryogenesis in medaka, Oryzias latipes.

Purpose: Zebrafish, a small fish model, exhibits a multipotent ability for retinal regeneration after damage throughout its lifetime. Compared with zebrafish, birds and mammals exhibit such a regenerative capacity only during the embryonic period, and this capacity decreases with age. In medaka, another small fish model that has also been used extensively in biological research, the retina's inner nuclear layer (INL) failed to regenerate after injury in the hatchling at eight days postfertilization (dpf). We characterized the regenerative process of the embryonic retina when the retinal injury occurred during the early embryonic period in medaka.

Methods: We employed a 10 Gy dose of gamma-ray irradiation to initiate retinal injury in medaka embryos at 3 dpf and performed histopathological analyses up to 21 dpf.

Results: One day after irradiation, numerous apoptotic neurons were observed in the INL; however, these neurons were rarely observed in the ciliary marginal zone and the photoreceptor layer. Numerous pyknotic cells were clustered in the irradiated retina until two days after irradiation. These disappeared four days after irradiation, but the abnormal bridging structures between the INL and ganglion cell layer (GCL) were present until 11 days after irradiation, and the neural layers were completely regenerated 18 days after irradiation. After gamma-ray irradiation, the spindle-like Müller glial cells in the INL became rounder but did not lose their ability to express SOX2.

Conclusions: Irradiated retina at 3 dpf of medaka embryos could be completely regenerated at 18 days after irradiation (21 dpf), although the abnormal layer structures bridging the INL and GCL were transiently formed in the retinas of all the irradiated embryos. Four days after irradiation, embryonic medaka Müller glia were reduced in number but maintained SOX2 expression as in nonirradiated embryos. This finding contrasts with previous reports that 8 dpf medaka larvae could not fully regenerate damaged retinas because of loss of SOX2 expression.

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来源期刊
CiteScore
5.00
自引率
11.50%
发文量
142
审稿时长
3 months
期刊介绍: The International Journal of Radiation Biology publishes original papers, reviews, current topic articles, technical notes/reports, and meeting reports on the effects of ionizing, UV and visible radiation, accelerated particles, electromagnetic fields, ultrasound, heat and related modalities. The focus is on the biological effects of such radiations: from radiation chemistry to the spectrum of responses of living organisms and underlying mechanisms, including genetic abnormalities, repair phenomena, cell death, dose modifying agents and tissue responses. Application of basic studies to medical uses of radiation extends the coverage to practical problems such as physical and chemical adjuvants which improve the effectiveness of radiation in cancer therapy. Assessment of the hazards of low doses of radiation is also considered.
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