纳米结构N/S掺杂碳点/介孔二氧化硅纳米颗粒和PVA复合水凝胶的制备及其抗微生物和抗生物膜应用

IF 5.2 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Pisut Pongchaikul , Tasnim Hajidariyor , Navarat Khetlai , Yu-Sheng Yu , Pariyapat Arjfuk , Pongtanawat Khemthong , Wanwitoo Wanmolee , Pattaraporn Posoknistakul , Navadol Laosiripojana , Kevin C.-W. Wu , Chularat Sakdaronnarong
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引用次数: 1

摘要

关于全球流行病的趋同,细菌感染的出现导致细菌病原体与一种或多种抗生素对抗,数量急剧增加。将工程纳米结构颗粒作为抗菌剂的递送载体是一种很有前途的方法,理论上可以克服上述挫折。在所有纳米颗粒中,已经发现二氧化硅纳米颗粒提供了有利于对抗细菌传染的功能特征。除此之外,碳点作为一种零维纳米材料,最近表现出了其光响应特性,可以产生活性氧,有助于增强微生物的抑制和灭活能力。在本研究中,研究了核/壳介孔二氧化硅纳米结构(MSN)与碳点(CDs)结合对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌的抗菌活性。与MSN偶联的氮和硫掺杂的CDs(NS/CDs)是一种具有成本效益的纳米颗粒,对所有测试细菌的抗菌活性是银纳米颗粒的4倍。在所有测试的纳米颗粒中,0.40 M NS/CDs@MSN在非常低的浓度(<0.125 mg mL−1)下显示出最大的最小生物膜抑制作用,其次是0.20 M NS/CDs@MSN(0.5 mg mL−1),CD@MSN(25 mg mL−1)和MSN(50 mg mL−2)。NS的固定化/CDs@MSN制备了聚乙烯醇(PVA)水凝胶,并进一步研究了其对成纤维细胞(NIH/3T3和L-929)的抗菌活性、生物膜控制效率和细胞毒性的影响,以供进一步的生物医学应用。结果表明,0.40 M NS/CDs-MSN@PVA水凝胶对金黄色葡萄球菌表现出最高的抑制作用>;第页。铜绿假单胞菌>;E。大肠杆菌。此外,MTT分析显示0.40M NS具有一定程度的毒性/CDs-MSN@PVA水凝胶对抗L-929细胞,当在0.40M NS的提取物中孵育时,细胞活力从100%略微降低到81.6%/CDs-MSN@PVA而未检测到相同水凝胶提取物对NIH/3T3细胞的毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Nanostructured N/S doped carbon dots/mesoporous silica nanoparticles and PVA composite hydrogel fabrication for anti-microbial and anti-biofilm application

Nanostructured N/S doped carbon dots/mesoporous silica nanoparticles and PVA composite hydrogel fabrication for anti-microbial and anti-biofilm application

Regarding the convergence of the worldwide epidemic, the appearance of bacterial infection has occasioned in a melodramatic upsurge in bacterial pathogens with confrontation against one or numerous antibiotics. The implementation of engineered nanostructured particles as a delivery vehicle for antimicrobial agent is one promising approach that could theoretically battle the setbacks mentioned. Among all nanoparticles, silica nanoparticles have been found to provide functional features that are advantageous for combatting bacterial contagion. Apart from that, carbon dots, a zero-dimension nanomaterial, have recently exhibited their photo-responsive property to generate reactive oxygen species facilitating to enhance microorganism suppression and inactivation ability. In this study, potentials of core/shell mesoporous silica nanostructures (MSN) in conjugation with carbon dots (CDs) toward antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli have been investigated. Nitrogen and sulfur doped CDs (NS/CDs) conjugated with MSN which were cost effective nanoparticles exhibited much superior antimicrobial activity for 4 times as much as silver nanoparticles against all bacteria tested. Among all nanoparticles tested, 0.40 M NS/CDs@MSN showed the greatest minimal biofilm inhibitory at very low concentration (< 0.125 mg mL−1), followed by 0.20 M NS/CDs@MSN (0.5 mg mL−1), CD@MSN (25 mg mL−1), and MSN (50 mg mL−1), respectively. Immobilization of NS/CDs@MSN in polyvinyl alcohol (PVA) hydrogel was performed and its effect on antimicrobial activity, biofilm controlling efficiency, and cytotoxicity toward fibroblast (NIH/3 T3 and L-929) cells was additionally studied for further biomedical applications. The results demonstrated that 0.40 M NS/CDs-MSN@PVA hydrogel exhibited the highest inhibitory effect on S. aureus > P. aeruginosa > E. coli. In addition, MTT assay revealed some degree of toxicity of 0.40 M NS/CDs-MSN@PVA hydrogel against L-929 cells by a slight reduction of cell viability from 100% to 81.6% when incubated in the extract from 0.40 M NS/CDs-MSN@PVA hydrogel, while no toxicity of the same hydrogel extract was detected toward NIH/3 T3 cells.

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来源期刊
International Journal of Pharmaceutics: X
International Journal of Pharmaceutics: X Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
6.60
自引率
0.00%
发文量
32
审稿时长
24 days
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