Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago
{"title":"尼古丁促进结核分枝杆菌H37Rv的生长和毒力基因的过表达","authors":"Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago","doi":"10.1111/1348-0421.13085","DOIUrl":null,"url":null,"abstract":"<p>Tobacco consumption increases the susceptibility to develop infectious diseases such as tuberculosis (TB). Nicotine (Nc) is the main component of cigarette smoke with immunomodulatory properties, however, its effect on <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) has been scarcely investigated. The present study evaluated the effect of nicotine on the growth of <i>Mtb</i> and on the induction of virulence-related genes. Mycobacteria were exposed to different concentrations of nicotine then <i>Mtb</i> growth was evaluated. Subsequently, the expression of the virulence-related genes <i>lysX, pirG, fad26, fbpa, ompa, hbhA, esxA, esxB, hspx, katG, lpqh</i>, and <i>caeA</i> was evaluated by RT-qPCR. The effect of nicotine on intracellular <i>Mtb</i> was also evaluated. The results showed that nicotine promotes the growth of <i>Mtb</i> both extracellularly and intracellularly and increases the expression of genes related to virulence. In summary, nicotine promotes the growth of <i>Mtb</i> and the expression of virulence-related genes that could be correlated with the increased the risk of smokers developing TB.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Nicotine promotes Mycobacterium tuberculosis H37Rv growth and overexpression of virulence genes\",\"authors\":\"Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago\",\"doi\":\"10.1111/1348-0421.13085\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Tobacco consumption increases the susceptibility to develop infectious diseases such as tuberculosis (TB). Nicotine (Nc) is the main component of cigarette smoke with immunomodulatory properties, however, its effect on <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) has been scarcely investigated. The present study evaluated the effect of nicotine on the growth of <i>Mtb</i> and on the induction of virulence-related genes. Mycobacteria were exposed to different concentrations of nicotine then <i>Mtb</i> growth was evaluated. Subsequently, the expression of the virulence-related genes <i>lysX, pirG, fad26, fbpa, ompa, hbhA, esxA, esxB, hspx, katG, lpqh</i>, and <i>caeA</i> was evaluated by RT-qPCR. The effect of nicotine on intracellular <i>Mtb</i> was also evaluated. The results showed that nicotine promotes the growth of <i>Mtb</i> both extracellularly and intracellularly and increases the expression of genes related to virulence. In summary, nicotine promotes the growth of <i>Mtb</i> and the expression of virulence-related genes that could be correlated with the increased the risk of smokers developing TB.</p>\",\"PeriodicalId\":18679,\"journal\":{\"name\":\"Microbiology and Immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-06-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology and Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.13085\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology and Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.13085","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Nicotine promotes Mycobacterium tuberculosis H37Rv growth and overexpression of virulence genes
Tobacco consumption increases the susceptibility to develop infectious diseases such as tuberculosis (TB). Nicotine (Nc) is the main component of cigarette smoke with immunomodulatory properties, however, its effect on Mycobacterium tuberculosis (Mtb) has been scarcely investigated. The present study evaluated the effect of nicotine on the growth of Mtb and on the induction of virulence-related genes. Mycobacteria were exposed to different concentrations of nicotine then Mtb growth was evaluated. Subsequently, the expression of the virulence-related genes lysX, pirG, fad26, fbpa, ompa, hbhA, esxA, esxB, hspx, katG, lpqh, and caeA was evaluated by RT-qPCR. The effect of nicotine on intracellular Mtb was also evaluated. The results showed that nicotine promotes the growth of Mtb both extracellularly and intracellularly and increases the expression of genes related to virulence. In summary, nicotine promotes the growth of Mtb and the expression of virulence-related genes that could be correlated with the increased the risk of smokers developing TB.
期刊介绍：
Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses.
Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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