Seo Gu Han, Hyuk Cheol Kwon, Do Hyun Kim, Seong Joon Hong, Sung Gu Han
{"title":"酸奶中Nisin和乳酸对幽门螺杆菌和人胃细胞的体外协同抗菌和抗炎作用。","authors":"Seo Gu Han, Hyuk Cheol Kwon, Do Hyun Kim, Seong Joon Hong, Sung Gu Han","doi":"10.5851/kosfa.2023.e34","DOIUrl":null,"url":null,"abstract":"<p><p><i>Helicobacter pylori</i> is a bacterium that naturally thrives in acidic environments and has the potential to induce various gastrointestinal disorders in humans. The antibiotic therapy utilized for treating <i>H. pylori</i> can lead to undesired side effects, such as dysbiosis in the gut microbiota. The objective of our study was to explore the potential antibacterial effects of nisin and lactic acid (LA) in yogurt against <i>H. pylori</i>. Additionally, we investigated the anti-inflammatory effects of nisin and LA in human gastric (AGS) cells infected with <i>H. pylori</i>. Nisin and LA combination showed the strongest inhibitory activity, with confirmed synergy at 0.375 fractional inhibitory concentration index. Also, post-fermented yogurt with incorporation of nisin exhibited antibacterial effect against <i>H. pylori</i>. The combination of nisin and LA resulted in a significant reduction of mRNA levels of bacterial toxins of <i>H. pylori</i> and pro-inflammatory cytokines in AGS cells infected with <i>H. pylori</i>. Furthermore, this also increased bacterial membrane damage, which led to DNA and protein leakage in <i>H. pylori</i>. Overall, the combination of nisin and LA shows promise as an alternative therapy for <i>H. pylori</i> infection. Additionally, the incorporation of nisin into foods containing LA presents a potential application. Further studies, including animal research, are needed to validate these findings and explore clinical applications.</p>","PeriodicalId":12459,"journal":{"name":"Food Science of Animal Resources","volume":"43 5","pages":"751-766"},"PeriodicalIF":4.2000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/fc/kosfa-43-5-751.PMC10493562.pdf","citationCount":"0","resultStr":"{\"title\":\"<i>In Vitro</i> Synergistic Antibacterial and Anti-Inflammatory Effects of Nisin and Lactic Acid in Yogurt against <i>Helicobacter pylori</i> and Human Gastric Cells.\",\"authors\":\"Seo Gu Han, Hyuk Cheol Kwon, Do Hyun Kim, Seong Joon Hong, Sung Gu Han\",\"doi\":\"10.5851/kosfa.2023.e34\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Helicobacter pylori</i> is a bacterium that naturally thrives in acidic environments and has the potential to induce various gastrointestinal disorders in humans. The antibiotic therapy utilized for treating <i>H. pylori</i> can lead to undesired side effects, such as dysbiosis in the gut microbiota. The objective of our study was to explore the potential antibacterial effects of nisin and lactic acid (LA) in yogurt against <i>H. pylori</i>. Additionally, we investigated the anti-inflammatory effects of nisin and LA in human gastric (AGS) cells infected with <i>H. pylori</i>. Nisin and LA combination showed the strongest inhibitory activity, with confirmed synergy at 0.375 fractional inhibitory concentration index. Also, post-fermented yogurt with incorporation of nisin exhibited antibacterial effect against <i>H. pylori</i>. The combination of nisin and LA resulted in a significant reduction of mRNA levels of bacterial toxins of <i>H. pylori</i> and pro-inflammatory cytokines in AGS cells infected with <i>H. pylori</i>. Furthermore, this also increased bacterial membrane damage, which led to DNA and protein leakage in <i>H. pylori</i>. Overall, the combination of nisin and LA shows promise as an alternative therapy for <i>H. pylori</i> infection. Additionally, the incorporation of nisin into foods containing LA presents a potential application. Further studies, including animal research, are needed to validate these findings and explore clinical applications.</p>\",\"PeriodicalId\":12459,\"journal\":{\"name\":\"Food Science of Animal Resources\",\"volume\":\"43 5\",\"pages\":\"751-766\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/fc/kosfa-43-5-751.PMC10493562.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Science of Animal Resources\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.5851/kosfa.2023.e34\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Science of Animal Resources","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5851/kosfa.2023.e34","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
In Vitro Synergistic Antibacterial and Anti-Inflammatory Effects of Nisin and Lactic Acid in Yogurt against Helicobacter pylori and Human Gastric Cells.
Helicobacter pylori is a bacterium that naturally thrives in acidic environments and has the potential to induce various gastrointestinal disorders in humans. The antibiotic therapy utilized for treating H. pylori can lead to undesired side effects, such as dysbiosis in the gut microbiota. The objective of our study was to explore the potential antibacterial effects of nisin and lactic acid (LA) in yogurt against H. pylori. Additionally, we investigated the anti-inflammatory effects of nisin and LA in human gastric (AGS) cells infected with H. pylori. Nisin and LA combination showed the strongest inhibitory activity, with confirmed synergy at 0.375 fractional inhibitory concentration index. Also, post-fermented yogurt with incorporation of nisin exhibited antibacterial effect against H. pylori. The combination of nisin and LA resulted in a significant reduction of mRNA levels of bacterial toxins of H. pylori and pro-inflammatory cytokines in AGS cells infected with H. pylori. Furthermore, this also increased bacterial membrane damage, which led to DNA and protein leakage in H. pylori. Overall, the combination of nisin and LA shows promise as an alternative therapy for H. pylori infection. Additionally, the incorporation of nisin into foods containing LA presents a potential application. Further studies, including animal research, are needed to validate these findings and explore clinical applications.
期刊介绍:
Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed journal publishing original research and review articles on scientific and technological aspects of chemistry, biotechnology, processing, engineering, and microbiology of meat, egg, dairy, and edible insect/worm products.