一种基于网络药理学的方法,探讨二氢杨梅素通过调节PPARG和CASP3对非酒精性脂肪肝大鼠的影响。

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Lu Liu , Sen Sun , Xiaohua Li
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引用次数: 0

摘要

背景:非酒精性脂肪肝(NAFLD)是我国最常见的肝病,目前尚无有效的治疗方法。这项工作旨在确认DHM在体内/体外的作用,并基于基于网络药理学的方法探索潜在的机制。方法:采用高脂饮食(HFD)饲养大鼠,以积累脂质。用不同浓度的DHM治疗HFD大鼠。用ELISA试剂盒检测血清总胆固醇(TC)、丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)。通过在线数据库筛选DHM抗NAFLD的靶基因。然后,用MTT法测定DHM对原代肝细胞和HepG2细胞的细胞毒性。qRT-PCR用于定量PPAGR和CASP3 mRNA的表达水平。检测细胞凋亡和细胞内甘油三酯(TG)。结果:HFD能提高大鼠肝重/体重比、血清TC、ALT和AST。但是DHM治疗可以减少这些升高的指标。DHM靶向NAFLD中的14个潜在基因。PPARG和CASP3是DHM对抗NAFLD的两个枢纽基因,评分因子系数分别为-7.1和-6.8 kcal/mol。DHM降低了棕榈酸诱导的PPARG mRNA水平和细胞内TG的升高。DHM可以降低棕榈酸诱导的CASP3 mRNA水平的升高和细胞凋亡。结论:本工作证明了DHM缓解脂质代谢紊乱和细胞凋亡的机制,为DHM在NAFLD中的潜在应用提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A network pharmacology-based approach to explore the effect of dihydromyricetin on non-alcoholic fatty liver rats via regulating PPARG and CASP3

Background

Non-alcohol fatty liver disease (NAFLD) is the most prevalent hepatopathy in China, with few effective cures currently. This work aimed to confirm the effect of DHM in vivo/vitro and explore the potential mechanism based on a network pharmacology-based approach.

Methods

The rats were fed using a high-fat diet (HFD) to accumulate lipid. DHM at different concentrations was used to treat the HFD rats. The serum total cholesterol (TC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were detected using ELISA kits. The target genes of DHM against NAFLD were screened by online databases. Then, the cytotoxicity of DHM in primary hepatocytes and HepG2 cells was determined by MTT reagent. qRT-PCR was used to quantify the expression level of PPAGR and CASP3 mRNA. Cell apoptosis and intracellular triglyceride (TG) were detected.

Results

HFD diet increased rat liver weight/body weight ratio, serum TC, ALT, and AST. But DHM treatment can reduce these elevated indicators. DHM targeted 14 potential genes in NAFLD. PPARG and CASP3 were two hub genes for DHM against NAFLD, with score factor coefficients of −7.1 and −6.8 kcal/mol. DHM reduced the increased PPARG mRNA level and intracellular TG induced by palmitic acid. DHM can reduce the increased CASP3 mRNA level and cell apoptosis induced by palmitic acid.

Conclusion

This work demonstrates a mechanism of DHM that alleviates lipid metabolism disorder and cell apoptosis for the treatment of NAFLD, evidencing the potential application of DHM in NAFLD.

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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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