推进体外毒理学的高通量筛选:成就、挑战和未来方向》。

IF 11.2 1区 医学 Q1 PHARMACOLOGY & PHARMACY
Caitlin Lynch, Srilatha Sakamuru, Masato Ooka, Ruili Huang, Carleen Klumpp-Thomas, Paul Shinn, David Gerhold, Anna Rossoshek, Sam Michael, Warren Casey, Michael F Santillo, Suzanne Fitzpatrick, Russell S Thomas, Anton Simeonov, Menghang Xia
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引用次数: 0

摘要

传统上,化学物质的毒性是通过体内动物实验来确定的,这种实验通量低、成本高,有时还无法预测化合物对人体的毒性。由于正在使用的化学品数量不断增加,而候选药物因毒性而失败的比例很高,因此开发体外高通量筛选方法来确定毒性势在必行。Tox21 计划是一个由联邦公共卫生机构组成的独特研究联盟,其成立的目的是利用一系列体外检测方法,以高通量、浓度响应的方式解决和确定毒性问题。在这篇文章中,我们回顾了高通量机器人筛选方法和信息学流程在生成毒理学数据方面取得的进步及其对该领域的影响;此外,我们还讨论了利用高效、可扩展的方法评估环境毒性的未来,这些方法能更好地代表人类相应的生物和毒效学过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-Throughput Screening to Advance In Vitro Toxicology: Accomplishments, Challenges, and Future Directions.

Traditionally, chemical toxicity is determined by in vivo animal studies, which are low throughput, expensive, and sometimes fail to predict compound toxicity in humans. Due to the increasing number of chemicals in use and the high rate of drug candidate failure due to toxicity, it is imperative to develop in vitro, high-throughput screening methods to determine toxicity. The Tox21 program, a unique research consortium of federal public health agencies, was established to address and identify toxicity concerns in a high-throughput, concentration-responsive manner using a battery of in vitro assays. In this article, we review the advancements in high-throughput robotic screening methodology and informatics processes to enable the generation of toxicological data, and their impact on the field; further, we discuss the future of assessing environmental toxicity utilizing efficient and scalable methods that better represent the corresponding biological and toxicodynamic processes in humans.

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来源期刊
CiteScore
27.80
自引率
0.00%
发文量
53
期刊介绍: Since 1961, the Annual Review of Pharmacology and Toxicology has been a comprehensive resource covering significant developments in pharmacology and toxicology. The journal encompasses various aspects, including receptors, transporters, enzymes, chemical agents, drug development science, and systems like the immune, nervous, gastrointestinal, cardiovascular, endocrine, and pulmonary systems. Special topics are also featured in this annual review.
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