甜味受体的T1R3亚基被D2O以跨膜结构域依赖的方式激活。

IF 2.8 4区心理学 Q1 BEHAVIORAL SCIENCES

Chemical Senses Pub Date : 2023-01-01 DOI:10.1093/chemse/bjad032

Natalie Ben Abu, Yaron Ben Shoshan-Galeczki, Einav Malach, Masha Y Niv

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引用次数: 0

摘要

氧化氘（D2O）是一种水，其中较重且稀有的同位素氘取代了两个氢。我们之前已经表明，D2O具有明显的甜味，由T1R2/T1R3甜味受体介导。在这里，我们探讨了重水对T1R2和T1R3亚基的影响。我们发现D2O激活T1R3转染的HEK293T细胞，类似于T1R2/T1R3转染的细胞。对溶解在D2O中的葡萄糖的反应高于在水中的反应。T1R3跨膜结构域7305.40位的苯丙氨酸突变为丙氨酸、亮氨酸或酪氨酸，损害或减少D2O的激活，表明T1R3 TMD结构域在传递重水信号中起着关键作用。

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The T1R3 subunit of the sweet taste receptor is activated by D2O in transmembrane domain-dependent manner.

Deuterium oxide (D2O) is water in which the heavier and rare isotope deuterium replaces both hydrogens. We have previously shown that D2O has a distinctly sweet taste, mediated by the T1R2/T1R3 sweet taste receptor. Here, we explore the effect of heavy water on T1R2 and T1R3 subunits. We show that D2O activates T1R3-transfected HEK293T cells similarly to T1R2/T1R3-transfected cells. The response to glucose dissolved in D2O is higher than in water. Mutations of phenylalanine at position 7305.40 in the transmembrane domain of T1R3 to alanine, leucine, or tyrosine impair or diminish activation by D2O, suggesting a critical role for T1R3 TMD domain in relaying the heavy water signal.

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来源期刊

Chemical Senses 医学-行为科学

CiteScore

8.60

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Chemical Senses publishes original research and review papers on all aspects of chemoreception in both humans and animals. An important part of the journal''s coverage is devoted to techniques and the development and application of new methods for investigating chemoreception and chemosensory structures.