多组学分析揭示了乌头二萜生物碱生物合成途径的进化起源。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Dake Zhao, Ya Zhang, Huanxing Ren, Yana Shi, Ding Dong, Zonghang Li, Guanghong Cui, Yong Shen, Zongmin Mou, Edward J. Kennelly, Luqi Huang, Jue Ruan, Suiyun Chen, Diqiu Yu, Yupeng Cun
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引用次数: 0

摘要

二萜生物碱(DA)由于其镇痛和抗炎特性,在临床实践中经常被使用。天然DA在毛茛科中普遍存在,尤其是在乌头属中。然而,负责DA产生的生物合成途径的进化起源仍然未知。在这项研究中,我们成功地组装了富含DA的Aconitum vilmorininum(a.vilmorinianum)(5.76 Gb)。使用比较基因组分析发现了一个A.vilmorinianum特异性的全基因组复制事件,这可能有助于DA生物合成途径的进化。我们通过综合基因组、转录组和代谢组学分析鉴定了几个参与DA生物合成的基因。这些基因包括编码靶戊烯氧化酶和氨基转移酶的酶,它们促进二萜的活化和氮原子插入二萜骨架,从而介导二萜转化为DA。进一步评估了这些基因在A.vilmorinianum中的分化期,结果表明,两种主要类型的基因参与了DA生物合成途径的建立。我们的综合分析为A.vilmorinianum中DAs的进化起源提供了新的见解,并为设计生物合成途径以获得所需的DAs提供了建议。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Multi-omics analysis reveals the evolutionary origin of diterpenoid alkaloid biosynthesis pathways in Aconitum

Multi-omics analysis reveals the evolutionary origin of diterpenoid alkaloid biosynthesis pathways in Aconitum

Diterpenoid alkaloids (DAs) have been often utilized in clinical practice due to their analgesic and anti-inflammatory properties. Natural DAs are prevalent in the family Ranunculaceae, notably in the Aconitum genus. Nevertheless, the evolutionary origin of the biosynthesis pathway responsible for DA production remains unknown. In this study, we successfully assembled a high-quality, pseudochromosome-level genome of the DA-rich species Aconitum vilmorinianum (A. vilmorinianum) (5.76 Gb). An A. vilmorinianum-specific whole-genome duplication event was discovered using comparative genomic analysis, which may aid in the evolution of the DA biosynthesis pathway. We identified several genes involved in DA biosynthesis via integrated genomic, transcriptomic, and metabolomic analyses. These genes included enzymes encoding target ent-kaurene oxidases and aminotransferases, which facilitated the activation of diterpenes and insertion of nitrogen atoms into diterpene skeletons, thereby mediating the transformation of diterpenes into DAs. The divergence periods of these genes in A. vilmorinianum were further assessed, and it was shown that two major types of genes were involved in the establishment of the DA biosynthesis pathway. Our integrated analysis offers fresh insights into the evolutionary origin of DAs in A. vilmorinianum as well as suggestions for engineering the biosynthetic pathways to obtain desired DAs.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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