登革热病毒 NS3 螺旋酶功能特性的热力学和机理分析。

IF 4.9 Q1 BIOPHYSICS

Biophysical reviews Pub Date : 2023-08-17 eCollection Date: 2023-08-01 DOI:10.1007/s12551-023-01101-5

J Jeremías Incicco, Leila A Cababie, Carolina Sarto, Natalia S Adler, Fernando Amrein, Evelyn Mikkelsen, Mehrnoosh Arrar, Sergio B Kaufman

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引用次数: 0

摘要

登革病毒（DENV）非结构蛋白 3（NS3）是一种多功能蛋白，对病毒的生命周期至关重要。DENV NS3 由丝氨酸蛋白酶结构域和螺旋酶结构域组成。螺旋酶结构域本身充当分子马达，沿着单链 RNA 以单向方式进行转运，或将双链 RNA 解旋，这一过程由三磷酸核苷的水解提供动力。在这篇简短的综述中，我们总结了我们在揭示 DENV NS3 作为 NTPase 和螺旋酶的热力学和机理功能特性方面所做的贡献和正在进行的努力。

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Thermodynamic and mechanistic analysis of the functional properties of dengue virus NS3 helicase.

The Dengue Virus (DENV) non-structural protein 3 (NS3) is a multi-functional protein critical in the viral life cycle. The DENV NS3 is comprised of a serine protease domain and a helicase domain. The helicase domain itself acts as a molecular motor, either translocating in a unidirectional manner along single-stranded RNA or unwinding double-stranded RNA, processes fueled by the hydrolysis of nucleoside triphosphates. In this brief review, we summarize our contributions and ongoing efforts to uncover the thermodynamic and mechanistic functional properties of the DENV NS3 as an NTPase and helicase.

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来源期刊

Biophysical reviews Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

8.90

自引率

0.00%

发文量

期刊介绍： Biophysical Reviews aims to publish critical and timely reviews from key figures in the field of biophysics. The bulk of the reviews that are currently published are from invited authors, but the journal is also open for non-solicited reviews. Interested authors are encouraged to discuss the possibility of contributing a review with the Editor-in-Chief prior to submission. Through publishing reviews on biophysics, the editors of the journal hope to illustrate the great power and potential of physical techniques in the biological sciences, they aim to stimulate the discussion and promote further research and would like to educate and enthuse basic researcher scientists and students of biophysics. Biophysical Reviews covers the entire field of biophysics, generally defined as the science of describing and defining biological phenomenon using the concepts and the techniques of physics. This includes but is not limited by such areas as: - Bioinformatics - Biophysical methods and instrumentation - Medical biophysics - Biosystems - Cell biophysics and organization - Macromolecules: dynamics, structures and interactions - Single molecule biophysics - Membrane biophysics, channels and transportation