Mohammad Reza Ghasemi, Akram Ranjbar, Pari Tamri, Shabnam Pourmoslemi, Alireza Nourian, Dara Dastan
{"title":"千叶阿喀琉叶精油对大鼠体外抗菌活性及伤口愈合作用的研究。","authors":"Mohammad Reza Ghasemi, Akram Ranjbar, Pari Tamri, Shabnam Pourmoslemi, Alireza Nourian, Dara Dastan","doi":"10.3831/KPI.2023.26.2.167","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>In this study we aimed to evaluate the <i>in vitro</i> antibacterial activity and wound healing properties of <i>Achillea millefolium</i> essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> using the broth dilution method.</p><p><strong>Methods: </strong>The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment.</p><p><strong>Results: </strong>Our results revealed the bacteriostatic activity of AMEO against <i>S. aureus</i> and <i>P. aeruginosa</i>. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group.</p><p><strong>Conclusion: </strong>The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 2","pages":"167-174"},"PeriodicalIF":1.2000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/a2/jop-26-2-167.PMC10315885.pdf","citationCount":"1","resultStr":"{\"title\":\"<i>In vitro</i> Antibacterial Activity and Wound Healing Effects of <i>Achillea millefolium</i> Essential Oil in Rat.\",\"authors\":\"Mohammad Reza Ghasemi, Akram Ranjbar, Pari Tamri, Shabnam Pourmoslemi, Alireza Nourian, Dara Dastan\",\"doi\":\"10.3831/KPI.2023.26.2.167\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>In this study we aimed to evaluate the <i>in vitro</i> antibacterial activity and wound healing properties of <i>Achillea millefolium</i> essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> using the broth dilution method.</p><p><strong>Methods: </strong>The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment.</p><p><strong>Results: </strong>Our results revealed the bacteriostatic activity of AMEO against <i>S. aureus</i> and <i>P. aeruginosa</i>. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group.</p><p><strong>Conclusion: </strong>The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.</p>\",\"PeriodicalId\":16769,\"journal\":{\"name\":\"Journal of Pharmacopuncture\",\"volume\":\"26 2\",\"pages\":\"167-174\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/a2/jop-26-2-167.PMC10315885.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pharmacopuncture\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3831/KPI.2023.26.2.167\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pharmacopuncture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3831/KPI.2023.26.2.167","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
In vitro Antibacterial Activity and Wound Healing Effects of Achillea millefolium Essential Oil in Rat.
Objectives: In this study we aimed to evaluate the in vitro antibacterial activity and wound healing properties of Achillea millefolium essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa using the broth dilution method.
Methods: The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment.
Results: Our results revealed the bacteriostatic activity of AMEO against S. aureus and P. aeruginosa. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group.
Conclusion: The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.
期刊介绍：
The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.
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