单链模板修复:提高基因编辑效率的关键见解。

IF 1.8 4区生物学 Q3 GENETICS & HEREDITY

Current Genetics Pub Date : 2021-10-01 DOI:10.1007/s00294-021-01186-z

Danielle N Gallagher, James E Haber

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引用次数: 9

摘要

DNA双链断裂(DSBs)对基因组的稳定性构成严重威胁。crispr - cas9介导的基因编辑有意创建位点特异性DSB来修改基因组序列，通常来自引入的单链DNA供体。然而，与典型的同源重组形式不同，单链模板修复(SSTR)是不依赖于rad51的。此外，该途径不同于其他先前表征的不依赖于rad51的过程。在这里，我们简要回顾了表征这一途径的工作，以及这些发现如何用于指导和改进当前的基因编辑策略。

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Single-strand template repair: key insights to increase the efficiency of gene editing.

DNA double-strand breaks (DSBs) pose a serious hazard for the stability of the genome. CRISPR-Cas9-mediated gene editing intentionally creates a site-specific DSB to modify the genomic sequence, typically from an introduced single-stranded DNA donor. However, unlike typical forms of homologous recombination, single-strand template repair (SSTR) is Rad51-independent. Moreover, this pathway is distinct from other previously characterized Rad51-independent processes. Here, we briefly review the work characterizing this pathway, and how these findings can be used to guide and improve current gene editing strategies.

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来源期刊

Current Genetics 生物-遗传学

CiteScore

6.00

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Current Genetics publishes genetic, genomic, molecular and systems-level analysis of eukaryotic and prokaryotic microorganisms and cell organelles. All articles are peer-reviewed. The journal welcomes submissions employing any type of research approach, be it analytical (aiming at a better understanding), applied (aiming at practical applications), synthetic or theoretical. Current Genetics no longer accepts manuscripts describing the genome sequence of mitochondria/chloroplast of a small number of species. Manuscripts covering sequence comparisons and analyses that include a large number of species will still be considered.