cAMP刺激家蚕前胸腺蜕皮甾体发生的信号传导

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
Shi-Hong Gu, Pei-Ling Lin
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引用次数: 0

摘要

在本研究中,我们研究了家蚕前胸腺(PG)中环磷酸腺苷(cAMP)信号传导的下游途径(与促胸激素(PTTH)刺激的蜕皮甾体发生有关)。结果表明,用二丁基cAMP(dbcAMP)或1-甲基-3-异丁基黄嘌呤(MIX)处理可抑制腺苷5′-单磷酸激活蛋白激酶(AMPK)的磷酸化,并激活翻译阻遏物4E-结合蛋白(4E-BP)的磷酸,4E-BP是雷帕霉素(TOR)信号传导的靶点标记物。AMPK的化学激活剂(5-氨基咪唑-4-羧酰胺-1-β-d-呋喃核糖糖苷,AICAR)增加了dbcAMP,抑制了AMPK磷酸化,并阻断了dbcAMP刺激的4E-BP磷酸化,表明对AMPK磷酸的抑制位于dbcAMP刺激的TOR信号传导的上游。用dbcAMP和MIX处理PGs也刺激了蛋白激酶C(PKC)底物抗体识别的37kDa蛋白的磷酸化,表明cAMP激活PKC信号传导。LY294002或AICAR处理均不影响dbcAMP刺激的PKC依赖性37kDa蛋白的磷酸化,表明cAMP刺激PKC信号传导与磷酸肌醇3-激酶(PI3K)或AMPK无关。此外,用LY294002、AICAR或钙磷素C预处理可部分抑制dbcAMP刺激的PGs中的蜕皮甾体生成。从这些结果中,我们得出结论,AMPK/TOR/4E-BP和PKC途径参与了由cAMP信号刺激的家蚕PGs的蜕皮素生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Signaling in cAMP-stimulated ecdysteroidogenesis in prothoracic glands of the silkworm, Bombyx mori

Signaling in cAMP-stimulated ecdysteroidogenesis in prothoracic glands of the silkworm, Bombyx mori

In the present study, we investigated downstream pathways of cyclic adenosine monophosphate (cAMP) signaling (which is related to prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis) in Bombyx mori prothoracic glands (PGs). Results showed that treatment with either dibutyryl cAMP (dbcAMP) or 1-methyl-3-isobutylxanthine (MIX) inhibited phosphorylation of adenosine 5′-monophosphate-activated protein kinase (AMPK) and activated phosphorylation of the translational repressor, 4E-binding protein (4E-BP), a marker of target of rapamycin (TOR) signaling. A chemical activator of AMPK (5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside, AICAR) increased dbcAMP-inhibited AMPK phosphorylation and blocked dbcAMP-stimulated phosphorylation of 4E-BP, indicating that inhibition of AMPK phosphorylation lies upstream of dbcAMP-stimulated TOR signaling. Treatment of PGs with dbcAMP and MIX also stimulated phosphorylation of a 37-kDa protein, as recognized by a protein kinase C (PKC) substrate antibody, indicating that cAMP activates PKC signaling. Treatment with either LY294002 or AICAR did not affect dbcAMP-stimulated phosphorylation of the PKC-dependent 37-kDa protein, indicating that cAMP-stimulated PKC signaling is not related to phosphoinositide 3-kinase (PI3K) or AMPK. In addition, dbcAMP-stimulated ecdysteroidogenesis in PGs was partially inhibited by pretreatment with either LY294002, AICAR, or calphostin C. From these results, we concluded that AMPK/TOR/4E-BP and PKC pathways are involved in ecdysteroidogenesis of PGs stimulated by cAMP signaling in B. mori.

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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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