ATG3 蛋白具有独特的两性α螺旋，对 Atg8/LC3 脂化反应至关重要。

IF 14.6 1区生物学 Q1 CELL BIOLOGY

Autophagy Pub Date : 2024-01-01 Epub Date: 2023-09-07 DOI:10.1080/15548627.2023.2255458

Taki Nishimura, Gianmarco Lazzeri, Sharon A Tooze, Roberto Covino

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引用次数: 0

摘要

在我们最近的论文中，我们发现 ATG3 在自噬膜上表现出很大程度的结构动态性，从而有效地进行 LC3 脂化。ATG3 蛋白具有一个由少量笨重和疏水残基识别的两性α-螺旋（AH）。这种生物物理指纹可使 ATG3 与膜瞬时结合，并促进其酶反应。这项研究将为从结构和机理上理解 ATG 蛋白在自噬体形成过程中如何与膜结合铺平道路。

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ATG3 proteins possess a unique amphipathic α-helix essential for the Atg8/LC3 lipidation reaction.

In our recent paper, we uncovered that ATG3 exhibits a large degree of structural dynamics on autophagic membranes to efficiently carry out LC3 lipidation. ATG3 proteins possess an amphipathic α-helix (AH) identified by a small number of bulky and hydrophobic residues. This biophysical fingerprint allows for transient membrane association of ATG3 and facilitates its enzymatic reaction. This study will pave the way for a structural and mechanistic understanding of how membrane association of ATG proteins is orchestrated during autophagosome formation.

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来源期刊

Autophagy 生物-细胞生物学

CiteScore

21.30

自引率

2.30%

发文量

277

审稿时长

1 months

期刊介绍： Autophagy is a peer-reviewed journal that publishes research on autophagic processes, including the lysosome/vacuole dependent degradation of intracellular material. It aims to be the premier journal in the field and covers various connections between autophagy and human health and disease, such as cancer, neurodegeneration, aging, diabetes, myopathies, and heart disease. Autophagy is interested in all experimental systems, from yeast to human. Suggestions for specialized topics are welcome. The journal accepts the following types of articles: Original research, Reviews, Technical papers, Brief Reports, Addenda, Letters to the Editor, Commentaries and Views, and Articles on science and art. Autophagy is abstracted/indexed in Adis International Ltd (Reactions Weekly), EBSCOhost (Biological Abstracts), Elsevier BV (EMBASE and Scopus), PubMed, Biological Abstracts, Science Citation Index Expanded, Web of Science, and MEDLINE.