Emily Sullivan, Maria D Macias Jimenez, Nicholas M Moore
{"title":"利用免疫层析横向流动法快速检测革兰氏阴性杆菌碳青霉烯酶的产生。","authors":"Emily Sullivan, Maria D Macias Jimenez, Nicholas M Moore","doi":"10.1093/labmed/lmad090","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Rapid detection of carbapenemase production in gram negative bacilli has important treatment considerations.</p><p><strong>Objective: </strong>We evaluated a lateral flow assay (LFA) for carbapenemase production compared with molecular detection of 5 (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48) carbapenemase genes.</p><p><strong>Methods: </strong>A total of 218 carbapenem nonsusceptible strains, including species of Enterobacterales, Pseudomonas aeruginosa isolated from clinical cultures were tested using the Cepheid Xpert Carba-R assay and the NG Biotech Carba-5 lateral flow immunoassay.</p><p><strong>Results: </strong>Overall agreement with LFA was 98.2% with accuracy for each target >99% compared with polymerase chain reaction. Results were available within 15 minutes compared with 1 hour for molecular detection.</p><p><strong>Conclusion: </strong>The use of accurate, rapid diagnostics compliments antimicrobial stewardship programs.</p>","PeriodicalId":17951,"journal":{"name":"Laboratory medicine","volume":" ","pages":"e204-e206"},"PeriodicalIF":0.0000,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Utilization of an immunochromatographic lateral flow assay for rapid detection of carbapenemase production in gram negative bacilli.\",\"authors\":\"Emily Sullivan, Maria D Macias Jimenez, Nicholas M Moore\",\"doi\":\"10.1093/labmed/lmad090\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Rapid detection of carbapenemase production in gram negative bacilli has important treatment considerations.</p><p><strong>Objective: </strong>We evaluated a lateral flow assay (LFA) for carbapenemase production compared with molecular detection of 5 (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48) carbapenemase genes.</p><p><strong>Methods: </strong>A total of 218 carbapenem nonsusceptible strains, including species of Enterobacterales, Pseudomonas aeruginosa isolated from clinical cultures were tested using the Cepheid Xpert Carba-R assay and the NG Biotech Carba-5 lateral flow immunoassay.</p><p><strong>Results: </strong>Overall agreement with LFA was 98.2% with accuracy for each target >99% compared with polymerase chain reaction. Results were available within 15 minutes compared with 1 hour for molecular detection.</p><p><strong>Conclusion: </strong>The use of accurate, rapid diagnostics compliments antimicrobial stewardship programs.</p>\",\"PeriodicalId\":17951,\"journal\":{\"name\":\"Laboratory medicine\",\"volume\":\" \",\"pages\":\"e204-e206\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Laboratory medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/labmed/lmad090\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laboratory medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/labmed/lmad090","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Utilization of an immunochromatographic lateral flow assay for rapid detection of carbapenemase production in gram negative bacilli.
Background: Rapid detection of carbapenemase production in gram negative bacilli has important treatment considerations.
Objective: We evaluated a lateral flow assay (LFA) for carbapenemase production compared with molecular detection of 5 (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48) carbapenemase genes.
Methods: A total of 218 carbapenem nonsusceptible strains, including species of Enterobacterales, Pseudomonas aeruginosa isolated from clinical cultures were tested using the Cepheid Xpert Carba-R assay and the NG Biotech Carba-5 lateral flow immunoassay.
Results: Overall agreement with LFA was 98.2% with accuracy for each target >99% compared with polymerase chain reaction. Results were available within 15 minutes compared with 1 hour for molecular detection.
Conclusion: The use of accurate, rapid diagnostics compliments antimicrobial stewardship programs.
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