海洋双壳类肺炎克雷伯菌质粒上临床相关抗生素和重金属抗性基因的共定位。

IF 3.9 3区 生物学 Q2 MICROBIOLOGY
MicrobiologyOpen Pub Date : 2023-07-19 DOI:10.1002/mbo3.1368
Fredrik Håkonsholm, Marit A. K. Hetland, Iren H. Löhr, Bjørn Tore Lunestad, Nachiket P. Marathe
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引用次数: 0

摘要

肺炎克雷伯菌是一种机会性病原体,经常与抗生素耐药性有关,存在于广泛的环境中,包括海洋栖息地。然而,人们对抗生素耐药性在这种环境中的发展、持续和传播知之甚少。本研究旨在获得从海洋双壳类中分离的抗生素抗性肺炎克雷伯菌的完整基因组序列,以确定这些分离物中抗生素和重金属抗性基因的遗传背景。使用Illumina MiSeq平台和Oxford Nanopore Technologies GridION设备,选择了五株抗生素耐药性肺炎克雷伯菌分离株进行全基因组测序,其中四株也携带重金属耐药性基因。进行缀合实验以检测所选质粒的转移潜力。完整基因组的平均长度为5.48 平均染色体大小为5.27的Mbp Mbp。在抗生素耐药菌株中检测到7个质粒。三个IncFIB、一个IncFIB/IncFII和一个IncFIB/IncHIB质粒分别携带抗生素抗性基因,如qnrS1、aph(6)-Id和aph(3')-Ia、aadA1和aadA2。其中四个质粒还携带编码对铜(pco)、银(sil)和砷(ars)抗性的基因。一个携带tet(D)和blaSHV-1以及pco、sil和ars基因的质粒通过接合转移到大肠杆菌中。我们展示了来自海洋双壳类肺炎克雷伯菌的偶联IncFIB质粒上抗生素和重金属抗性基因的共存。我们的研究强调了海洋环境和海鲜作为抗生素耐药性可能传播途径的重要性,并为重金属共同选择抗生素耐药性基因的潜力提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Co-localization of clinically relevant antibiotic- and heavy metal resistance genes on plasmids in Klebsiella pneumoniae from marine bivalves

Co-localization of clinically relevant antibiotic- and heavy metal resistance genes on plasmids in Klebsiella pneumoniae from marine bivalves

Klebsiella pneumoniae is an opportunistic pathogen frequently associated with antibiotic resistance and present in a wide range of environments, including marine habitats. However, little is known about the development, persistence, and spread of antibiotic resistance in such environments. This study aimed to obtain the complete genome sequences of antibiotic-resistant K. pneumoniae isolated from marine bivalves in order to determine the genetic context of antibiotic- and heavy metal resistance genes in these isolates. Five antibiotic-resistant K. pneumoniae isolates, of which four also carried heavy metal resistance genes, were selected for complete genome sequencing using the Illumina MiSeq platform and the Oxford Nanopore Technologies GridION device. Conjugation experiments were conducted to examine the transfer potential of selected plasmids. The average length of the complete genomes was 5.48 Mbp with a mean chromosome size of 5.27 Mbp. Seven plasmids were detected in the antibiotic-resistant isolates. Three IncFIB, one IncFIB/IncFII, and one IncFIB/IncHIB plasmid, respectively, carried antibiotic resistance genes such as qnrS1, aph(6)-Id and aph(3′)-Ia, aadA1, and aadA2. Four of these plasmids also carried genes encoding resistance to copper (pco), silver (sil), and arsenic (ars). One plasmid carrying tet(D) and blaSHV-1 as well as pco, sil, and ars genes was transferred to Escherichia coli by conjugation. We show the co-occurrence of antibiotic- and heavy metal resistance genes on a conjugative IncFIB plasmid from K. pneumoniae from marine bivalves. Our study highlights the importance of the marine environment and seafood as a possible dissemination route for antimicrobial resistance and provides insights into the potential for co-selection of antibiotic resistance genes by heavy metals.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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