BJ Moloney, M Deveney, K Ellard, P Hick, PD Kirkland, NJG Moody, J Frances
{"title":"2011年澳大利亚太平洋牡蛎(Magallana gigas, Thunberg, 1793)中Ostreid herpesvirus-1微变异监测","authors":"BJ Moloney, M Deveney, K Ellard, P Hick, PD Kirkland, NJG Moody, J Frances","doi":"10.1111/avj.13265","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To demonstrate that OsHV-1 microvariant was limited to the known infected areas in New South Wales at the time of the survey in 2011.</p>\n </section>\n \n <section>\n \n <h3> Design</h3>\n \n <p>A 2-stage survey to demonstrate probability of infection at 2% design prevalence within oyster growing regions and to detect at least one infected region (4% design prevalence) with 95% confidence.</p>\n </section>\n \n <section>\n \n <h3> Sample Population</h3>\n \n <p><i>Magallana gigas</i> in nominated oyster growing regions in New South Wales, South Australia and Tasmania as approved by the Aquatic Consultative Committee on Emergency Animal Diseases and documented in a national surveillance plan.</p>\n </section>\n \n <section>\n \n <h3> Procedure</h3>\n \n <p>Field sampling for active surveillance and laboratory selection of appropriate tissues using methods to minimize potential for cross contamination. Published methods for qPCR and conventional PCR for OsHV-1 microvariant. Stochastic analysis of survey results to demonstrate probability of detection in the areas tested.</p>\n </section>\n \n <section>\n \n <h3> Results and Conclusions</h3>\n \n <p>OsHV-1 microvariant was not detected in a total 4121 samples according to the case definition developed for the survey. However, in NSW a screening qPCR for OsHV-1 detected 13 samples that reacted. These samples were negative at 2 laboratories in the qPCR and conventional PCR assays used in the case definition for the survey. We concluded that oyster production areas of Australia outside the infected area in NSW met the criteria for self-declaration of freedom at the time of the survey in 2011.</p>\n </section>\n \n <section>\n \n <h3> Clinical Relevance</h3>\n \n <p>This activity illustrated achievements in surveillance for an emerging emergency animal pathogen where epidemiological and test validation data were limited, but where data was required to inform the emergency disease response. It also illustrated the challenges faced by investigators in interpreting surveillance results using tests with limited validation. It was guided by and has informed improvements in surveillance and emergency disease preparedness.</p>\n </section>\n </div>","PeriodicalId":8661,"journal":{"name":"Australian Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/avj.13265","citationCount":"0","resultStr":"{\"title\":\"Ostreid herpesvirus-1 microvariant surveillance in Pacific oysters (Magallana gigas, Thunberg, 1793) in Australia in 2011\",\"authors\":\"BJ Moloney, M Deveney, K Ellard, P Hick, PD Kirkland, NJG Moody, J Frances\",\"doi\":\"10.1111/avj.13265\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To demonstrate that OsHV-1 microvariant was limited to the known infected areas in New South Wales at the time of the survey in 2011.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Design</h3>\\n \\n <p>A 2-stage survey to demonstrate probability of infection at 2% design prevalence within oyster growing regions and to detect at least one infected region (4% design prevalence) with 95% confidence.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Sample Population</h3>\\n \\n <p><i>Magallana gigas</i> in nominated oyster growing regions in New South Wales, South Australia and Tasmania as approved by the Aquatic Consultative Committee on Emergency Animal Diseases and documented in a national surveillance plan.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Procedure</h3>\\n \\n <p>Field sampling for active surveillance and laboratory selection of appropriate tissues using methods to minimize potential for cross contamination. Published methods for qPCR and conventional PCR for OsHV-1 microvariant. Stochastic analysis of survey results to demonstrate probability of detection in the areas tested.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results and Conclusions</h3>\\n \\n <p>OsHV-1 microvariant was not detected in a total 4121 samples according to the case definition developed for the survey. However, in NSW a screening qPCR for OsHV-1 detected 13 samples that reacted. These samples were negative at 2 laboratories in the qPCR and conventional PCR assays used in the case definition for the survey. We concluded that oyster production areas of Australia outside the infected area in NSW met the criteria for self-declaration of freedom at the time of the survey in 2011.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Clinical Relevance</h3>\\n \\n <p>This activity illustrated achievements in surveillance for an emerging emergency animal pathogen where epidemiological and test validation data were limited, but where data was required to inform the emergency disease response. It also illustrated the challenges faced by investigators in interpreting surveillance results using tests with limited validation. It was guided by and has informed improvements in surveillance and emergency disease preparedness.</p>\\n </section>\\n </div>\",\"PeriodicalId\":8661,\"journal\":{\"name\":\"Australian Veterinary Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-07-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/avj.13265\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australian Veterinary Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/avj.13265\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Veterinary Journal","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/avj.13265","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Ostreid herpesvirus-1 microvariant surveillance in Pacific oysters (Magallana gigas, Thunberg, 1793) in Australia in 2011
Objective
To demonstrate that OsHV-1 microvariant was limited to the known infected areas in New South Wales at the time of the survey in 2011.
Design
A 2-stage survey to demonstrate probability of infection at 2% design prevalence within oyster growing regions and to detect at least one infected region (4% design prevalence) with 95% confidence.
Sample Population
Magallana gigas in nominated oyster growing regions in New South Wales, South Australia and Tasmania as approved by the Aquatic Consultative Committee on Emergency Animal Diseases and documented in a national surveillance plan.
Procedure
Field sampling for active surveillance and laboratory selection of appropriate tissues using methods to minimize potential for cross contamination. Published methods for qPCR and conventional PCR for OsHV-1 microvariant. Stochastic analysis of survey results to demonstrate probability of detection in the areas tested.
Results and Conclusions
OsHV-1 microvariant was not detected in a total 4121 samples according to the case definition developed for the survey. However, in NSW a screening qPCR for OsHV-1 detected 13 samples that reacted. These samples were negative at 2 laboratories in the qPCR and conventional PCR assays used in the case definition for the survey. We concluded that oyster production areas of Australia outside the infected area in NSW met the criteria for self-declaration of freedom at the time of the survey in 2011.
Clinical Relevance
This activity illustrated achievements in surveillance for an emerging emergency animal pathogen where epidemiological and test validation data were limited, but where data was required to inform the emergency disease response. It also illustrated the challenges faced by investigators in interpreting surveillance results using tests with limited validation. It was guided by and has informed improvements in surveillance and emergency disease preparedness.
期刊介绍:
Over the past 80 years, the Australian Veterinary Journal (AVJ) has been providing the veterinary profession with leading edge clinical and scientific research, case reports, reviews. news and timely coverage of industry issues. AJV is Australia''s premier veterinary science text and is distributed monthly to over 5,500 Australian Veterinary Association members and subscribers.