Milene Dias Miranda, Gabriela Cardoso Caldas, Vivian Neuza Ferreira, Ortrud Monika Barth, Aline de Paula Dias da Silva, Mayara Secco Torres Silva, Beatriz Grinsztejn, Valdiléa Gonçalves Veloso, Thiago Moreno Souza, Edson Elias da Silva, Debora Ferreira Barreto-Vieira
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引用次数: 0
摘要
背景：根据美国疾病控制和预防中心（CDC）最近发布的2023年猴痘（Mpox）疫情全球地图，有100多个国家没有报告猴痘感染病例。巴西在其中脱颖而出，成为上一次疫情中病例数最多的第二个国家：目的：帮助了解病毒在细胞模型中的感染效应，这对于诊断尚未纳入医疗服务提供者鉴别诊断的感染以及制定病毒抑制策略非常重要：方法：我们描述了从巴西一名患者的人体临床样本中分离病毒的方案，以及通过斑块形成单位（PFU）测定、逆转录酶聚合酶链反应（RT-PCR）和透射电子显微镜（TEM）在细胞模型中的病毒生长情况：我们从临床诊断的 Mpox 阳性样本的 Vero 细胞培养中分离病毒，并使用 TEM 显示感染对细胞结构的影响：主要结论：了解病毒生长对细胞结构的影响及其复制动力学可为开发具有抗病毒特性的新药提供更好的策略。
Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case.
Background: According to the last 2023 Monkeypox (Mpox) Outbreak Global Map from the Centres for Disease Control and Prevention (CDC), more than 100 countries with no Mpox infection report cases. Brazil stands out in this group and is the second country with the highest number of cases in the last outbreak.
Objective: To contribute to knowledge of the virus infection effects in a cellular model, which is important for diagnosis infections not yet included in a provider´s differential diagnosis and for developing viral inhibition strategies.
Methods: We describe a virus isolation protocol for a human clinical sample from a patient from Brazil, the viral growth in a cell model through plaque forming units (PFU) assay, reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM).
Findings: We follow the viral isolation in Vero cell culture from a Mpox positive clinically diagnosed sample and show the infection effects on cellular structures using a TEM.
Main conclusions: Understanding the impact of viral growth on cellular structures and its replication kinetics may offer better strategies for the development of new drugs with antiviral properties.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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