Filopodia体外和体内。

IF 11.4 1区 生物学 Q1 CELL BIOLOGY
Thomas C A Blake, Jennifer L Gallop
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引用次数: 1

摘要

Filopodia是用于细胞运动、病原体感染和组织发育的动态细胞表面突起。决定丝状足类生长和缩回的方式和位置的分子机制需要将机械力和膜弯曲与细胞外信号传导和细胞骨架的更广泛状态相结合。所涉及的肌动蛋白调节机制使肌动蛋白丝与下面的肌动蛋白皮层分开成核、伸长和成束。丝足的精细膜和肌动蛋白几何结构、组织背景的重要性、所需的高时空分辨率和高度冗余都限制了电流模型。新技术正在增加功能洞察的机会,从纯化的成分中体外重建丝状足类,内源性基因修饰,诱导型扰动系统,以及在多细胞环境中研究丝状足类。在这篇综述中,我们探讨了丝状伪足如何形成的概念模型、参与这一过程的分子的最新进展,以及我们对丝状伪足在体外和体内的最新理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Filopodia In Vitro and In Vivo.

Filopodia are dynamic cell surface protrusions used for cell motility, pathogen infection, and tissue development. The molecular mechanisms determining how and where filopodia grow and retract need to integrate mechanical forces and membrane curvature with extracellular signaling and the broader state of the cytoskeleton. The involved actin regulatory machinery nucleates, elongates, and bundles actin filaments separately from the underlying actin cortex. The refined membrane and actin geometry of filopodia, importance of tissue context, high spatiotemporal resolution required, and high degree of redundancy all limit current models. New technologies are improving opportunities for functional insight, with reconstitution of filopodia in vitro from purified components, endogenous genetic modification, inducible perturbation systems, and the study of filopodia in multicellular environments. In this review, we explore recent advances in conceptual models of how filopodia form, the molecules involved in this process, and our latest understanding of filopodia in vitro and in vivo.

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来源期刊
CiteScore
19.50
自引率
0.00%
发文量
21
期刊介绍: The Annual Review of Cell and Developmental Biology, established in 1985, comprehensively addresses major advancements in cell and developmental biology. Encompassing the structure, function, and organization of cells, as well as the development and evolution of cells in relation to both single and multicellular organisms, the journal explores models and tools of molecular biology. As of the current volume, the journal has transitioned from gated to open access through Annual Reviews' Subscribe to Open program, making all articles published under a CC BY license.
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