了解口腔生物膜复杂性的评估模型和评估技术。

IF 3.9 3区 生物学 Q2 MICROBIOLOGY
MicrobiologyOpen Pub Date : 2023-08-29 DOI:10.1002/mbo3.1377
Srinivas Sulugodu Ramachandra, Patricia Wright, Pingping Han, Abdalla Abdal-hay, Ryan S. B. Lee, Saso Ivanovski
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引用次数: 1

摘要

口腔生物膜是引发牙齿疾病的三维(3D)复杂实体,在科学文献中使用几种生物膜模型和评估技术对其进行了广泛评估。生物膜模型和评估技术的清单可能会让新手生物膜研究人员不知所措。这篇叙述性综述旨在总结关于生物膜模型和评估技术的现有文献,提供关于选择适当模型和相应评估技术的额外信息,这可能对初级生物膜研究者和经验丰富的研究人员有用。该综述介绍了以前建立的2D模型,根据生长环境、营养物质的可用性和细菌种类的数量概述了它们的优势和局限性,同时也探索了新的3D生物膜模型。讨论了生物膜在临床相关3D模型上的生长,特别是熔融电写纤维支架,重点是以前没有报道过的。综述了近年来备受关注的已验证口腔微观模型的相关研究。该综述分析了生物膜评估方法的优点和局限性,包括菌落形成单位培养、结晶紫、2,3-双-(2-甲氧基-4-硝基-5-磺基苯基)-2H-四氮唑-5-碳酰亚胺内盐分析、共聚焦显微镜、荧光原位杂交、扫描电子显微镜、定量聚合酶链式反应和下一代测序。根据设备/设施的可用性,使用更复杂的模型和先进的评估方法,可能有助于开发临床相关的生物膜并回答适当的研究问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluating models and assessment techniques for understanding oral biofilm complexity

Evaluating models and assessment techniques for understanding oral biofilm complexity

Oral biofilms are three-dimensional (3D) complex entities initiating dental diseases and have been evaluated extensively in the scientific literature using several biofilm models and assessment techniques. The list of biofilm models and assessment techniques may overwhelm a novice biofilm researcher. This narrative review aims to summarize the existing literature on biofilm models and assessment techniques, providing additional information on selecting an appropriate model and corresponding assessment techniques, which may be useful as a guide to the beginner biofilm investigator and as a refresher to experienced researchers. The review addresses previously established 2D models, outlining their advantages and limitations based on the growth environment, availability of nutrients, and the number of bacterial species, while also exploring novel 3D biofilm models. The growth of biofilms on clinically relevant 3D models, particularly melt electrowritten fibrous scaffolds, is discussed with a specific focus that has not been previously reported. Relevant studies on validated oral microcosm models that have recently gaining prominence are summarized. The review analyses the advantages and limitations of biofilm assessment methods, including colony forming unit culture, crystal violet, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt assays, confocal microscopy, fluorescence in situ hybridization, scanning electron microscopy, quantitative polymerase chain reaction, and next-generation sequencing. The use of more complex models with advanced assessment methodologies, subject to the availability of equipment/facilities, may help in developing clinically relevant biofilms and answering appropriate research questions.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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