用光学镊子对单个细菌和孢子进行物理化学表征

IF 2.5 4区 生物学 Q3 MICROBIOLOGY
Daniel P.G. Nilsson , Unni Lise Jonsmoen , Dmitry Malyshev , Rasmus Öberg , Krister Wiklund , Magnus Andersson
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引用次数: 0

摘要

孢子形成的病原菌适合粘附在表面,它们的内孔可以耐受使去污困难的强烈化学物质。因此,了解细菌和孢子的物理化学性质对于开发抗粘附表面和消毒技术至关重要。然而,大量测量物理化学性质并不能显示细胞之间的异质性。因此,在单细胞水平上表征细菌可以提供通常隐藏在大量测量中的机制线索。本文展示了如何应用光镊子来表征单个细菌和孢子,以及如何评估与粘附、流体动力学、生物化学和代谢活性相关的物理化学性质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physico-chemical characterization of single bacteria and spores using optical tweezers

Spore-forming pathogenic bacteria are adapted for adhering to surfaces, and their endospores can tolerate strong chemicals making decontamination difficult. Understanding the physico-chemical properties of bacteria and spores is therefore essential in developing antiadhesive surfaces and disinfection techniques. However, measuring physico-chemical properties in bulk does not show the heterogeneity between cells. Characterizing bacteria on a single-cell level can thereby provide mechanistic clues usually hidden in bulk measurements. This paper shows how optical tweezers can be applied to characterize single bacteria and spores, and how physico-chemical properties related to adhesion, fluid dynamics, biochemistry, and metabolic activity can be assessed.

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来源期刊
Research in microbiology
Research in microbiology 生物-微生物学
CiteScore
4.10
自引率
3.80%
发文量
54
审稿时长
16 days
期刊介绍: Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.
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