Alana A. S. Gonçalves, Camila A. Lopes, Marcelo A. Levenhagen, Henrique T. Gonzaga, Luiz Carlos M. Oliveira, Julia Maria Costa-Cruz
{"title":"多份粪便取样和特定的寄生虫学技术对诊断酒精中毒患者的类圆线虫病至关重要","authors":"Alana A. S. Gonçalves, Camila A. Lopes, Marcelo A. Levenhagen, Henrique T. Gonzaga, Luiz Carlos M. Oliveira, Julia Maria Costa-Cruz","doi":"10.1007/s11686-023-00700-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Chronic alcoholism is a well-known risk factor for strongyloidiasis, in these patients the disease is potentially more severe, probably due to the breakdown of local protective barriers and immunosuppression caused by alcohol, which can lead to autoinfection and dissemination. The aim of this study was to evaluate multiple stool sampling and a specific parasitological assay agar plate culture (APC) for the diagnosis of <i>Strongyloides stercoralis</i> in alcoholics.</p><h3>Methods</h3><p>APC was compared to sedimentation technique (HPJ; Hoffman, Pons and Janer), as parasitological methods to detect <i>S. stercoralis</i> infection in alcoholic individuals. Three stool samples from 60 alcoholic and 60 non-alcoholic individuals were analyzed.</p><h3>Results</h3><p><i>S. stercoralis</i> larvae were observed in 11 (18.3%) alcoholic individuals and 1 (1.7%) nonalcoholic individual (<i>P</i> = 0.0042). In view of the combined results, sensitivity for the APC method was 63.6% (CI 31.6–87.6%) with the first sample reaching 100% (CI 67.8–100%) after analyzing three fecal samples. The HPJ sensitivity was 36.4% (CI 12.4–68.4) in the first sample, reaching 72.7% (CI 39.3–92.7) after three samples analyzed.</p><h3>Conclusion</h3><p>The present results suggest that in alcoholic patients, it is important to repeat stool sampling with specific techniques, especially using the APC method, to avoid misdiagnosis in cases that could evolve to disseminated strongyloidiasis.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"68 3","pages":"718 - 722"},"PeriodicalIF":1.2000,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multiple Stool Sampling and Specific Parasitological Technique are Crucial to Diagnose Strongyloidiasis in Alcoholic Patients\",\"authors\":\"Alana A. S. Gonçalves, Camila A. Lopes, Marcelo A. Levenhagen, Henrique T. Gonzaga, Luiz Carlos M. Oliveira, Julia Maria Costa-Cruz\",\"doi\":\"10.1007/s11686-023-00700-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Chronic alcoholism is a well-known risk factor for strongyloidiasis, in these patients the disease is potentially more severe, probably due to the breakdown of local protective barriers and immunosuppression caused by alcohol, which can lead to autoinfection and dissemination. The aim of this study was to evaluate multiple stool sampling and a specific parasitological assay agar plate culture (APC) for the diagnosis of <i>Strongyloides stercoralis</i> in alcoholics.</p><h3>Methods</h3><p>APC was compared to sedimentation technique (HPJ; Hoffman, Pons and Janer), as parasitological methods to detect <i>S. stercoralis</i> infection in alcoholic individuals. Three stool samples from 60 alcoholic and 60 non-alcoholic individuals were analyzed.</p><h3>Results</h3><p><i>S. stercoralis</i> larvae were observed in 11 (18.3%) alcoholic individuals and 1 (1.7%) nonalcoholic individual (<i>P</i> = 0.0042). In view of the combined results, sensitivity for the APC method was 63.6% (CI 31.6–87.6%) with the first sample reaching 100% (CI 67.8–100%) after analyzing three fecal samples. The HPJ sensitivity was 36.4% (CI 12.4–68.4) in the first sample, reaching 72.7% (CI 39.3–92.7) after three samples analyzed.</p><h3>Conclusion</h3><p>The present results suggest that in alcoholic patients, it is important to repeat stool sampling with specific techniques, especially using the APC method, to avoid misdiagnosis in cases that could evolve to disseminated strongyloidiasis.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"68 3\",\"pages\":\"718 - 722\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-08-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-023-00700-2\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-023-00700-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Multiple Stool Sampling and Specific Parasitological Technique are Crucial to Diagnose Strongyloidiasis in Alcoholic Patients
Purpose
Chronic alcoholism is a well-known risk factor for strongyloidiasis, in these patients the disease is potentially more severe, probably due to the breakdown of local protective barriers and immunosuppression caused by alcohol, which can lead to autoinfection and dissemination. The aim of this study was to evaluate multiple stool sampling and a specific parasitological assay agar plate culture (APC) for the diagnosis of Strongyloides stercoralis in alcoholics.
Methods
APC was compared to sedimentation technique (HPJ; Hoffman, Pons and Janer), as parasitological methods to detect S. stercoralis infection in alcoholic individuals. Three stool samples from 60 alcoholic and 60 non-alcoholic individuals were analyzed.
Results
S. stercoralis larvae were observed in 11 (18.3%) alcoholic individuals and 1 (1.7%) nonalcoholic individual (P = 0.0042). In view of the combined results, sensitivity for the APC method was 63.6% (CI 31.6–87.6%) with the first sample reaching 100% (CI 67.8–100%) after analyzing three fecal samples. The HPJ sensitivity was 36.4% (CI 12.4–68.4) in the first sample, reaching 72.7% (CI 39.3–92.7) after three samples analyzed.
Conclusion
The present results suggest that in alcoholic patients, it is important to repeat stool sampling with specific techniques, especially using the APC method, to avoid misdiagnosis in cases that could evolve to disseminated strongyloidiasis.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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