纤维软骨干细胞驱动生长小鼠颞下颌关节骨性关节炎的不同软骨/成骨转导规律

IF 10.8 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Ruiye Bi, Qianli Li, Haohan Li, Peng Wang, Han Fang, Xianni Yang, Yiru Wang, Yi Hou, Binbin Ying, Songsong Zhu
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引用次数: 0

摘要

前盘移位(ADD)可导致青少年颞下颌关节骨性关节炎(TMJOA)和下颌发育迟缓。为了研究纤维软骨干细胞(FCSCs)在这一过程中的潜在功能作用,我们建立了ADD-TMJOA手术小鼠模型。从模型生成后1周开始,ADD小鼠下颌生长迟缓加重,伴骨关节炎(OA)样关节软骨变性,表现为软骨分化受损,软骨下骨稳态丧失。Gli1-CreER+的谱系追踪Tmfl/-小鼠和Sox9- creer +;Tmfl/-小鼠显示,ADD干扰Gli1+ FCSCs的成软骨能力以及Sox9+谱系的成骨分化,主要发生在TMJ软骨的中间区。然后,建立手术诱导的椎间盘复位(DR)小鼠模型。DR治疗可显著缓解adhd小鼠fscs的抑制能力。DR治疗后,adhd小鼠和青少年adhd患者OA表型均明显缓解,髁突生长明显改善。综上所述,ADD-TMJOA可导致软骨祖细胞能力和FCSCs谱系成骨分化受损,导致软骨退变和软骨下骨稳态丧失,最终导致TMJ生长迟缓。早期DR能明显缓解软骨退变,恢复TMJ软骨生长潜能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Divergent chondro/osteogenic transduction laws of fibrocartilage stem cell drive temporomandibular joint osteoarthritis in growing mice.

Divergent chondro/osteogenic transduction laws of fibrocartilage stem cell drive temporomandibular joint osteoarthritis in growing mice.

The anterior disc displacement (ADD) leads to temporomandibular joint osteoarthritis (TMJOA) and mandibular growth retardation in adolescents. To investigate the potential functional role of fibrocartilage stem cells (FCSCs) during the process, a surgical ADD-TMJOA mouse model was established. From 1 week after model generation, ADD mice exhibited aggravated mandibular growth retardation with osteoarthritis (OA)-like joint cartilage degeneration, manifesting with impaired chondrogenic differentiation and loss of subchondral bone homeostasis. Lineage tracing using Gli1-CreER+; Tmfl/-mice and Sox9-CreER+;Tmfl/-mice showed that ADD interfered with the chondrogenic capacity of Gli1+ FCSCs as well as osteogenic differentiation of Sox9+ lineage, mainly in the middle zone of TMJ cartilage. Then, a surgically induced disc reposition (DR) mouse model was generated. The inhibited FCSCs capacity was significantly alleviated by DR treatment in ADD mice. And both the ADD mice and adolescent ADD patients had significantly relieved OA phenotype and improved condylar growth after DR treatment. In conclusion, ADD-TMJOA leads to impaired chondrogenic progenitor capacity and osteogenesis differentiation of FCSCs lineage, resulting in cartilage degeneration and loss of subchondral bone homeostasis, finally causing TMJ growth retardation. DR at an early stage could significantly alleviate cartilage degeneration and restore TMJ cartilage growth potential.

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来源期刊
International Journal of Oral Science
International Journal of Oral Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
31.80
自引率
1.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: The International Journal of Oral Science covers various aspects of oral science and interdisciplinary fields, encompassing basic, applied, and clinical research. Topics include, but are not limited to: Oral microbiology Oral and maxillofacial oncology Cariology Oral inflammation and infection Dental stem cells and regenerative medicine Craniofacial surgery Dental material Oral biomechanics Oral, dental, and maxillofacial genetic and developmental diseases Craniofacial bone research Craniofacial-related biomaterials Temporomandibular joint disorder and osteoarthritis The journal publishes peer-reviewed Articles presenting new research results and Review Articles offering concise summaries of specific areas in oral science.
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