发酵乳酸杆菌和酿酒酵母菌对肉鸡生长性能、血液学性状、血清生化、粪便和肠道微生物群的影响

IF 6.8 4区 医学 Q1 NUTRITION & DIETETICS
Subrota Hati, Krupali Ramanuj, Bethsheba Basaiawmoit, Prakash Koringa, Mansi Desai, Dinesh J Ghodasara, Kuldip V Joshi, Mohsin Pathan, Sreeja V, Nikesh J Bhagora, Fulabhai P Savaliya, B K Mishra
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引用次数: 2

摘要

目的:通过分析肉鸡生产性能、血液学性状、血清生化、组织病理学、粪便细菌计数及盲肠宏基因组分析,在肉鸡饲料中添加乳酸菌和酵母替代免疫调节剂,实现无抗生素肉蛋生产。方法:采用KGL4(发酵乳酸杆菌MTCC 25515)和WBS2A(酿酒酵母菌GI: MG101828)两种培养物,在家禽饲料中不添加免疫调节剂和商业益生菌的情况下,对肉鸡的健康和生长进行了42天的研究。96日龄肉鸡分为:T1[对照组:基础饲粮+免疫调节因子和商品益生菌]、T2[不含免疫调节因子和商品益生菌的基础饲粮+ KGL4 (108 CFU/mL)]、T3[不含免疫调节因子和商品益生菌的基础饲粮+ WBS2A (107 CFU/mL)]和T4[不含免疫调节因子和商品益生菌的基础饲粮+ KGL4 + WBS2A,比例为1:1](机构动物伦理委员会(IAEC) 365/PRS/2022号)。测定肉鸡盲肠样品的增重、饲料消耗比(FCR)、白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白含量(WBC)、血小板计数(RBC)、胆固醇含量、甘油三酯、高密度脂蛋白(HDL)、极低密度脂蛋白(VLDL)、粪便计数及宏基因组分析。结果:在各项性状中,与对照组相比,经发酵乳酸杆菌(KGL4)处理组的整体性能有所提高。发酵乳酸杆菌(KGL4)处理组体增重(2583.04±35.421 g)、FCR(1.60±0.019)、WBC(235.60±2.562 × 103/µL)、血红蛋白(14.10±0.442 g/dl)、HDL(131.40±11.400 mg/dl)显著高于对照组。在对盲肠样本进行宏基因组分析时,调查未显示不同类群间属或门的相对比例有显著差异。血液学特性也有改善;心脏、肠和肝组织无坏死迹象。结论:本研究表明,在肉鸡饲料中添加发酵乳酸杆菌和酿酒酵母菌是安全的,也支持了目前关于酵母和乳酸菌作为一种有效的替代兴奋剂来维持肉鸡健康和生长的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Significance of Limosilactobacillus fermentum and Saccharomyces cerevisiae on the Growth Performance, Haematological Traits, Serum Biochemistry, Faecal and Caeca Microbiota of Broiler Chickens.

Objective: The aim of the study was to supplement Lactobacillus and yeast in broiler feed by replacing immunomodulators to develop antibiotic free meat and egg production by analyzing broiler performance, haematological traits, serum biochemistry, histopathology, fecal bacterial count, and metagenomic analysis of broiler ceca.

Method: Two cultures i.e. KGL4 (Limosilactobacillus fermentum MTCC 25515) and WBS2A (Saccharomyces cerevisiae GI: MG101828) were considered for the evaluation of Broiler chicken's health and growth during 42 days study without supplementing immunomodulators and commercial probiotics in poultry feeds. The 96-day-old broiler chickens were grouped into: T1 [Control: basal diet + immunomodulatory factor and commercial probiotic], T2 [Basal diet without immunomodulatory factor and commercial probiotic + KGL4 (108 CFU/mL), T3 [Basal diet without immunomodulatory factor and commercial probiotic + WBS2A (107 CFU/mL), and T4 [Basal diet without immunomodulatory factor and commercial probiotic + KGL4 + WBS2A in a 1:1 ratio] (Institutional Animal Ethics Committee (IAEC) No. 365/PRS/2022). The following parameters, i.e., body weight gain, feed consumption ratio (FCR), white blood cell count (WBC), red blood cell count (RBC), hemoglobin content, platelet count, cholesterol content, triglycerides, high density lipoprotein (HDL), very low-density lipoprotein (VLDL), fecal counts and metagenomic analysis of broiler ceca samples, were measured.

Results: In the study, amongst various traits, the overall performance of the group treated along with Limosilactobacillus fermentum (KGL4) showed improved results as compared to control group. Limosilactobacillus fermentum (KGL4) treated group had higher body weight gain (2583.04 ± 35.421 g), FCR (1.60 ± 0.019), WBC (235.60 ± 2.562 × 103/µL), hemoglobin content (14.10 ± 0.442 g/dl), and HDL (131.40 ± 11.400 mg/dl). The investigation did not show significant variations in the relative proportions of genus or phylum among various groups during metagenomic analysis of ceca samples. There was also an improvement in haematological traits; no evidence of necrosis in heart, intestine and liver tissues.

Conclusions: The present study conclude that it is safe to feed Limosilactobacillus fermentum and Saccharomyces cerevisiae to broilers as feed supplements and also supports the current knowledge regarding the use of yeast and lactic acid bacteria as an effective alternative stimulant for maintaining health and growth of broiler chickens.

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