Ricu Claassens , Stephanus N. Venter , Chrizelle W. Beukes , Tomasz Stępkowski , Wai Y. Chan , Emma T. Steenkamp
{"title":"Bradyrhizobium xenoudolans sp.nov.从入侵南非的澳大利亚Acacia物种的根瘤中分离得到。","authors":"Ricu Claassens , Stephanus N. Venter , Chrizelle W. Beukes , Tomasz Stępkowski , Wai Y. Chan , Emma T. Steenkamp","doi":"10.1016/j.syapm.2023.126452","DOIUrl":null,"url":null,"abstract":"<div><p>A genealogical concordance approach was used to delineate strains isolated from <em>Acacia dealbata</em> and <em>Acacia mearnsii</em> root nodules in South Africa. These isolates form part of <em>Bradyrhizobium</em> based on 16S rRNA sequence similarity. Phylogenetic analysis of six housekeeping genes (<em>atpD, dnaK, glnII, gyrB, recA</em> and <em>rpoB</em>) confirmed that these isolates represent a novel species, while pairwise average nucleotide identity (ANIb) calculations with the closest type strains (<em>B. cosmicum</em> 58S1<sup>T</sup>, <em>B. betae</em> PL7HG1<sup>T</sup>, <em>B. ganzhouense</em> CCBAU 51670 <sup>T</sup>, <em>B. cytisi</em> CTAW11<sup>T</sup> and <em>B. rifense</em> CTAW71<sup>T</sup>) resulted in values well below 95–96%. We further performed phenotypic tests which revealed that there are high levels of intraspecies variation, while an additional analysis of the <em>nodA</em> and <em>nifD</em> loci indicated that the symbiotic loci of the strains are closely related to those of <em>Bradyrhizobium</em> isolates with an Australian origin. Strain 14AB<sup>T</sup> (=LMG 31415 <sup>T</sup> = SARCC-753 <sup>T</sup>) is designated as the type strain of the novel species for which we propose the name <em>Bradyrhizobium xenonodulans</em> sp. nov.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 5","pages":"Article 126452"},"PeriodicalIF":3.3000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Bradyrhizobium xenonodulans sp. nov. isolated from nodules of Australian Acacia species invasive to South Africa\",\"authors\":\"Ricu Claassens , Stephanus N. Venter , Chrizelle W. Beukes , Tomasz Stępkowski , Wai Y. Chan , Emma T. Steenkamp\",\"doi\":\"10.1016/j.syapm.2023.126452\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A genealogical concordance approach was used to delineate strains isolated from <em>Acacia dealbata</em> and <em>Acacia mearnsii</em> root nodules in South Africa. These isolates form part of <em>Bradyrhizobium</em> based on 16S rRNA sequence similarity. Phylogenetic analysis of six housekeeping genes (<em>atpD, dnaK, glnII, gyrB, recA</em> and <em>rpoB</em>) confirmed that these isolates represent a novel species, while pairwise average nucleotide identity (ANIb) calculations with the closest type strains (<em>B. cosmicum</em> 58S1<sup>T</sup>, <em>B. betae</em> PL7HG1<sup>T</sup>, <em>B. ganzhouense</em> CCBAU 51670 <sup>T</sup>, <em>B. cytisi</em> CTAW11<sup>T</sup> and <em>B. rifense</em> CTAW71<sup>T</sup>) resulted in values well below 95–96%. We further performed phenotypic tests which revealed that there are high levels of intraspecies variation, while an additional analysis of the <em>nodA</em> and <em>nifD</em> loci indicated that the symbiotic loci of the strains are closely related to those of <em>Bradyrhizobium</em> isolates with an Australian origin. Strain 14AB<sup>T</sup> (=LMG 31415 <sup>T</sup> = SARCC-753 <sup>T</sup>) is designated as the type strain of the novel species for which we propose the name <em>Bradyrhizobium xenonodulans</em> sp. nov.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"46 5\",\"pages\":\"Article 126452\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202023000619\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000619","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Bradyrhizobium xenonodulans sp. nov. isolated from nodules of Australian Acacia species invasive to South Africa
A genealogical concordance approach was used to delineate strains isolated from Acacia dealbata and Acacia mearnsii root nodules in South Africa. These isolates form part of Bradyrhizobium based on 16S rRNA sequence similarity. Phylogenetic analysis of six housekeeping genes (atpD, dnaK, glnII, gyrB, recA and rpoB) confirmed that these isolates represent a novel species, while pairwise average nucleotide identity (ANIb) calculations with the closest type strains (B. cosmicum 58S1T, B. betae PL7HG1T, B. ganzhouense CCBAU 51670 T, B. cytisi CTAW11T and B. rifense CTAW71T) resulted in values well below 95–96%. We further performed phenotypic tests which revealed that there are high levels of intraspecies variation, while an additional analysis of the nodA and nifD loci indicated that the symbiotic loci of the strains are closely related to those of Bradyrhizobium isolates with an Australian origin. Strain 14ABT (=LMG 31415 T = SARCC-753 T) is designated as the type strain of the novel species for which we propose the name Bradyrhizobium xenonodulans sp. nov.
期刊介绍：
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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