模拟胃肠道炭疽病。

IF 2.5 4区 生物学 Q3 MICROBIOLOGY
So Young Oh , Alice Château , Anastasia Tomatsidou , Derek Elli, Haley Gula, Olaf Schneewind , Dominique Missiakas
{"title":"模拟胃肠道炭疽病。","authors":"So Young Oh ,&nbsp;Alice Château ,&nbsp;Anastasia Tomatsidou ,&nbsp;Derek Elli,&nbsp;Haley Gula,&nbsp;Olaf Schneewind ,&nbsp;Dominique Missiakas","doi":"10.1016/j.resmic.2023.104026","DOIUrl":null,"url":null,"abstract":"<div><p><span><em>Bacillus anthracis</em></span><span> is a spore-forming microbe that persists in soil and causes anthrax disease. The most natural route of infection is ingestion<span> by grazing animals. Gastrointestinal (GI) anthrax also occurs in their monogastric predators, including humans. Exposure of carcasses<span> to oxygen triggers sporulation and contamination of the surrounding soil completing the unusual life cycle of this microbe. The pathogenesis of GI anthrax is poorly characterized. Here, we use </span></span></span><em>B. anthracis</em><span> carrying the virulence plasmids<span> pXO1 and pXO2, to model gastrointestinal disease in Guinea pigs and mice. We find that spores germinate in the GI tract and precipitate disease in a dose-dependent manner. Inoculation of vegetative bacilli also results in GI anthrax. Virulence is impacted severely by the loss of capsule (pXO2-encoded) but only moderately in absence of toxins (pXO1-encoded). Nonetheless, the lack of toxins leads to reduced bacterial replication in infected hosts. </span></span><em>B. cereus</em> Elc4, a strain isolated from a fatal case of inhalational anthrax-like disease, was also found to cause GI anthrax. Because transmission to new hosts depends on the release of large numbers of spores in the environment, we propose that the acquisition of pXO1- and pXO2-like plasmids may promote the successful expansion of members of the <span><em>Bacillus cereus</em><em> sensu lato</em></span> group able to cause anthrax-like disease.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338639/pdf/","citationCount":"2","resultStr":"{\"title\":\"Modeling gastrointestinal anthrax disease\",\"authors\":\"So Young Oh ,&nbsp;Alice Château ,&nbsp;Anastasia Tomatsidou ,&nbsp;Derek Elli,&nbsp;Haley Gula,&nbsp;Olaf Schneewind ,&nbsp;Dominique Missiakas\",\"doi\":\"10.1016/j.resmic.2023.104026\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><em>Bacillus anthracis</em></span><span> is a spore-forming microbe that persists in soil and causes anthrax disease. The most natural route of infection is ingestion<span> by grazing animals. Gastrointestinal (GI) anthrax also occurs in their monogastric predators, including humans. Exposure of carcasses<span> to oxygen triggers sporulation and contamination of the surrounding soil completing the unusual life cycle of this microbe. The pathogenesis of GI anthrax is poorly characterized. Here, we use </span></span></span><em>B. anthracis</em><span> carrying the virulence plasmids<span> pXO1 and pXO2, to model gastrointestinal disease in Guinea pigs and mice. We find that spores germinate in the GI tract and precipitate disease in a dose-dependent manner. Inoculation of vegetative bacilli also results in GI anthrax. Virulence is impacted severely by the loss of capsule (pXO2-encoded) but only moderately in absence of toxins (pXO1-encoded). Nonetheless, the lack of toxins leads to reduced bacterial replication in infected hosts. </span></span><em>B. cereus</em> Elc4, a strain isolated from a fatal case of inhalational anthrax-like disease, was also found to cause GI anthrax. Because transmission to new hosts depends on the release of large numbers of spores in the environment, we propose that the acquisition of pXO1- and pXO2-like plasmids may promote the successful expansion of members of the <span><em>Bacillus cereus</em><em> sensu lato</em></span> group able to cause anthrax-like disease.</p></div>\",\"PeriodicalId\":21098,\"journal\":{\"name\":\"Research in microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10338639/pdf/\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0923250823000013\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0923250823000013","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 2

摘要

炭疽杆菌是一种孢子形成微生物,能在土壤中持续存在并引起炭疽病。最自然的感染途径是被放牧动物摄入。胃肠道炭疽也发生在包括人类在内的单胃捕食者身上。尸体暴露在氧气中会引发孢子形成和周围土壤的污染,从而完成这种微生物不同寻常的生命周期。胃肠道炭疽病的发病机制尚不明确。在这里,我们使用携带毒力质粒pXO1和pXO2的炭疽杆菌来模拟豚鼠和小鼠的胃肠道疾病。我们发现孢子在胃肠道中发芽并以剂量依赖的方式引发疾病。接种营养杆菌也会导致胃肠道炭疽病。胶囊(pXO2编码)的缺失会严重影响病毒性,但在没有毒素(pXO1编码)的情况下只会适度影响病毒性。尽管如此,毒素的缺乏会导致受感染宿主中细菌复制的减少。蜡状芽孢杆菌Elc4,一种从致命的吸入性炭疽样疾病中分离出来的菌株,也被发现会引起胃肠道炭疽。由于传播到新宿主取决于环境中大量孢子的释放,我们提出,pXO1-和pXO2-样质粒的获得可能促进能够引起炭疽样疾病的蜡样芽孢杆菌群成员的成功扩增。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modeling gastrointestinal anthrax disease

Bacillus anthracis is a spore-forming microbe that persists in soil and causes anthrax disease. The most natural route of infection is ingestion by grazing animals. Gastrointestinal (GI) anthrax also occurs in their monogastric predators, including humans. Exposure of carcasses to oxygen triggers sporulation and contamination of the surrounding soil completing the unusual life cycle of this microbe. The pathogenesis of GI anthrax is poorly characterized. Here, we use B. anthracis carrying the virulence plasmids pXO1 and pXO2, to model gastrointestinal disease in Guinea pigs and mice. We find that spores germinate in the GI tract and precipitate disease in a dose-dependent manner. Inoculation of vegetative bacilli also results in GI anthrax. Virulence is impacted severely by the loss of capsule (pXO2-encoded) but only moderately in absence of toxins (pXO1-encoded). Nonetheless, the lack of toxins leads to reduced bacterial replication in infected hosts. B. cereus Elc4, a strain isolated from a fatal case of inhalational anthrax-like disease, was also found to cause GI anthrax. Because transmission to new hosts depends on the release of large numbers of spores in the environment, we propose that the acquisition of pXO1- and pXO2-like plasmids may promote the successful expansion of members of the Bacillus cereus sensu lato group able to cause anthrax-like disease.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Research in microbiology
Research in microbiology 生物-微生物学
CiteScore
4.10
自引率
3.80%
发文量
54
审稿时长
16 days
期刊介绍: Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信