克服单分子浓度障碍的策略。

IF 4.6 Q1 CHEMISTRY, ANALYTICAL
David S. White, Mackinsey A. Smith, Baron Chanda and Randall H. Goldsmith*, 
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引用次数: 2

摘要

基于荧光的单分子方法有助于彻底改变我们对化学和生物机制的理解。不幸的是,这些方法只适用于低浓度的荧光分子,因此感兴趣的单个荧光物种可以成功地分辨出背景信号之外的荧光。因此,尽管大多数生物和化学过程发生在高得多的反应物浓度下,但这些技术的应用仅限于高亲和力相互作用。幸运的是,最近的方法学进展表明,这种浓度屏障确实可以被打破,技术浓度高达1mM。这篇综述的目标是讨论在高浓度下进行单分子荧光技术的挑战,在生物学和化学中提供应用,并强调打破注意力障碍的主要里程碑。我们还希望激发这些技术的广泛应用,以便我们能够开始探索超越这一障碍的新物理现象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Strategies for Overcoming the Single-Molecule Concentration Barrier

Strategies for Overcoming the Single-Molecule Concentration Barrier

Fluorescence-based single-molecule approaches have helped revolutionize our understanding of chemical and biological mechanisms. Unfortunately, these methods are only suitable at low concentrations of fluorescent molecules so that single fluorescent species of interest can be successfully resolved beyond background signal. The application of these techniques has therefore been limited to high-affinity interactions despite most biological and chemical processes occurring at much higher reactant concentrations. Fortunately, recent methodological advances have demonstrated that this concentration barrier can indeed be broken, with techniques reaching concentrations as high as 1 mM. The goal of this Review is to discuss the challenges in performing single-molecule fluorescence techniques at high-concentration, offer applications in both biology and chemistry, and highlight the major milestones that shatter the concentration barrier. We also hope to inspire the widespread use of these techniques so we can begin exploring the new physical phenomena lying beyond this barrier.

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来源期刊
ACS Measurement Science Au
ACS Measurement Science Au 化学计量学-
CiteScore
5.20
自引率
0.00%
发文量
0
期刊介绍: ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.
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