七氟醚对乳腺癌金属蛋白酶和自然杀伤组2、成员D (NKG2D)配体表达及自然杀伤细胞介导的细胞毒性影响的体外研究

IF 4.2 4区 医学 Q1 ANESTHESIOLOGY
Korean Journal of Anesthesiology Pub Date : 2023-12-01 Epub Date: 2023-08-21 DOI:10.4097/kja.23323
Hyae Jin Kim, Soeun Jeon, Hyeon Jeong Lee, Jaeho Bae, Hyun-Su Ri, Jeong-Min Hong, Sung In Paek, Seul Ki Kwon, Jae-Rin Kim, Seungbin Park, Eun-Jung Yun
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引用次数: 0

摘要

背景:我们研究了七氟醚暴露对乳腺癌细胞基质金属蛋白酶(MMP)表达、自然杀伤组2、成员D (NKG2D)配体(ul16结合蛋白1-3和主要组织相容性复合体I类链相关分子A/B)的表达和消融以及自然杀伤(NK)细胞介导的细胞毒性的影响。方法:将3株人乳腺癌细胞株MCF-7、MDA-MB-453和HCC-70分别与0(对照)、600 (S6)和1200 μM (S12)七氟醚孵育4 h,采用多重聚合酶链式反应(PCR)和流式细胞术分别检测NKG2D配体基因表达和癌细胞表面蛋白表达。分别用western blotting和酶联免疫吸附法分析MMP-1和-2蛋白表达和可溶性NKG2D配体浓度。结果:七氟醚在MCF-7、MDA-MB-453和HCC-70细胞中下调NKG2D配体mRNA和蛋白的表达呈剂量依赖性,但不影响MCF-7、MDA-MB-453和HCC-70细胞中MMP-1和-2的表达以及可溶性NKG2D配体的浓度。七氟醚在MCF-7、MDA-MB-453和HCC-70细胞中以剂量依赖的方式减弱NK细胞介导的癌细胞裂解(P = 0.040、P = 0.040和P = 0.040)。结论:我们的研究结果表明,七氟醚暴露以剂量依赖的方式减弱NK细胞介导的乳腺癌细胞毒性。这可能归因于七氟醚诱导的NKG2D配体转录的减少,而不是七氟醚诱导的MMP表达及其蛋白水解活性的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of sevoflurane on metalloproteinase and natural killer group 2, member D (NKG2D) ligand expression and natural killer cell-mediated cytotoxicity in breast cancer: an in vitro study.

Background: We investigated the effects of sevoflurane exposure on the expression of matrix metalloproteinase (MMP), expression and ablation of natural killer group 2, member D (NKG2D) ligands (UL16-binding proteins 1-3 and major histocompatibility complex class I chain-related molecules A/B), and natural killer (NK) cell-mediated cytotoxicity in breast cancer cells.

Methods: Three human breast cancer cell lines (MCF-7, MDA-MB-453, and HCC-70) were incubated with 0 (control), 600 (S6), or 1200 μM (S12) sevoflurane for 4 h. The gene expression of NKG2D ligands and their protein expression on cancer cell surfaces were measured using multiplex polymerase chain reaction (PCR) and flow cytometry, respectively. Protein expression of MMP-1 and -2 and the concentration of soluble NKG2D ligands were analyzed using western blotting and enzyme-linked immunosorbent assays, respectively.

Results: Sevoflurane downregulated the mRNA and protein expression of the NKG2D ligand in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells but did not affect the expression of MMP-1 or -2 or the concentration of soluble NKG2D ligands in the MCF-7, MDA-MB-453, and HCC-70 cells. Sevoflurane attenuated NK cell-mediated cancer cell lysis in a dose-dependent manner in MCF-7, MDA-MB-453, and HCC-70 cells (P = 0.040, P = 0.040, and P = 0.040, respectively).

Conclusions: Our results demonstrate that sevoflurane exposure attenuates NK cell-mediated cytotoxicity in breast cancer cells in a dose-dependent manner. This could be attributed to a sevoflurane-induced decrease in the transcription of NKG2D ligands rather than sevoflurane-induced changes in MMP expression and their proteolytic activity.

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来源期刊
CiteScore
6.20
自引率
6.90%
发文量
84
审稿时长
16 weeks
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