NaCl诱导的高渗胁迫对荧光假单胞菌细胞表面特性的影响

Abd Alaziz Abu Quba, Marc-Oliver Goebel, Mariam Karagulyan, Anja Miltner, Matthias Kästner, Jörg Bachmann, Gabriele E Schaumann, Doerte Diehl
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引用次数: 1

摘要

测定水分胁迫对细菌表面特性的影响是研究细菌诱发土壤拒水性的关键。环境条件的变化会影响细菌的一些特性,如细胞的疏水性和形态。在此,我们研究了适应高渗胁迫对荧光假单胞菌细胞润湿性、形状、粘附性和表面化学成分的影响。由此,我们的目的是发现接触角研究的细菌膜的润湿性变化与原子和化学力显微镜(AFM, CFM)研究的单细胞之间可能存在的关系,这是目前尚缺乏的。我们表明,通过应力,细胞表面对疏水功能化探针的粘附力增加,而对亲水功能化尖端的粘附力减少。这与接触角的结果一致。此外,细胞大小缩小,蛋白质含量增加。结果提出了两种可能的机制:细胞收缩伴随着外膜囊泡的释放,从而使蛋白脂比增加。较高的蛋白质含量增加了硬度和每表面积疏水纳米结构域的数量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Changes in cell surface properties of <i>Pseudomonas fluorescens</i> by adaptation to NaCl induced hypertonic stress.

Changes in cell surface properties of <i>Pseudomonas fluorescens</i> by adaptation to NaCl induced hypertonic stress.

Changes in cell surface properties of <i>Pseudomonas fluorescens</i> by adaptation to NaCl induced hypertonic stress.

Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress.

Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area.

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CiteScore
3.30
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