电子受体和铵盐限制下麦卡蒂代盐球虫在混合培养中的长期存活。

Nadia Morson, Olivia Molenda, Katherine J Picott, Ruth E Richardson, Elizabeth A Edwards
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引用次数: 1

摘要

麦卡蒂脱卤球(Dehalococcoides mccartyi)的少数菌株携带并表达氯乙烯还原酶(VcrA),该酶催化氯乙烯(VC)的脱氯,氯乙烯是一种致癌的土壤和地下水污染物。vcrA操纵子是在基因组岛(GI)上发现的,因此被认为参与水平基因转移(HGT)。为了尝试诱导vcrA-GI的HGT,我们在不含铵的培养基中混合两种富集培养,同时提供VC。我们假设这些条件会选择一种既能固定氮又能呼吸VC的D. mccartyi突变株。然而,经过4年多的潜伏期,我们没有发现vcrA-GI的HGT证据。相反,我们观察到vc脱氯活性归因于三氯乙烯还原酶TceA。测序和蛋白质模型显示,预测的TceA活性位点发生突变,这可能影响了底物特异性。我们还在KB-1培养中发现了两株固氮D. mccartyi菌株。具有不同表型的多株麦卡蒂菌的存在是自然环境和某些富集培养物(如KB-1)的特征,并可能提高生物强化的成功率。事实上,多个不同的菌株在培养物中持续存在了几十年,而我们无法诱导vcrA-GI的HGT,这表明它不像预测的那样具有流动性,或者流动性在尚未发现的特定dehalococides亚支中受到限制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Long-term survival of <i>Dehalococcoides mccartyi</i> strains in mixed cultures under electron acceptor and ammonium limitation.

Long-term survival of <i>Dehalococcoides mccartyi</i> strains in mixed cultures under electron acceptor and ammonium limitation.

Long-term survival of <i>Dehalococcoides mccartyi</i> strains in mixed cultures under electron acceptor and ammonium limitation.

Long-term survival of Dehalococcoides mccartyi strains in mixed cultures under electron acceptor and ammonium limitation.

Few strains of Dehalococcoides mccartyi harbour and express the vinyl chloride reductase (VcrA) that catalyzes the dechlorination of vinyl chloride (VC), a carcinogenic soil and groundwater contaminant. The vcrA operon is found on a Genomic Island (GI) and, therefore, believed to participate in horizontal gene transfer (HGT). To try to induce HGT of the vcrA-GI, we blended two enrichment cultures in medium without ammonium while providing VC. We hypothesized that these conditions would select for a mutant strain of D. mccartyi that could both fix nitrogen and respire VC. However, after more than 4 years of incubation, we found no evidence for HGT of the vcrA-GI. Rather, we observed VC-dechlorinating activity attributed to the trichloroethene reductase TceA. Sequencing and protein modelling revealed a mutation in the predicted active site of TceA, which may have influenced substrate specificity. We also identified two nitrogen-fixing D. mccartyi strains in the KB-1 culture. The presence of multiple strains of D. mccartyi with distinct phenotypes is a feature of natural environments and certain enrichment cultures (such as KB-1), and may enhance bioaugmentation success. The fact that multiple distinct strains persist in the culture for decades and that we could not induce HGT of the vcrA-GI suggests that it is not as mobile as predicted, or that mobility is restricted in ways yet to be discovered to specific subclades of Dehalococcoides.

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来源期刊
CiteScore
3.30
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